Department of Chemical and Biological Engineering, Sookmyung Women's University , 100 Cheongpa-ro 47 gil, Yongsan-gu, Seoul 04310, Republic of Korea.
Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-ro, Nam-gu, Pohang 37673, Republic of Korea.
Inorg Chem. 2017 Nov 6;56(21):12859-12865. doi: 10.1021/acs.inorgchem.7b01684.
Challenges exist in taking advantage of dye molecules for reliable and reproducible molecular probes in biomedical applications. In this study, we show how to utilize the dye molecules for bioimaging within protective carriers of nanocrystalline metal-organic frameworks (nMOFs) particles. Specifically, Resorufin and Rhodamine-6G having different molecular sizes were encapsulated within close-fitting pores of nMOF-801 and nUiO-67 particles, respectively. The resulting nanocrystalline particles have high crystallinity, uniform size, and morphology and preserve enhanced photoluminescence properties with exceptional stabilities in biomedical environment. The samples are further functionalized with a targeting agent and successfully work for fluorescence imaging of FL83B (human hepatocyte cell) and HepG2 (human hepatocellular carcinoma) without cytotoxicity.
在将染料分子应用于生物医学应用中的可靠和可重复的分子探针时，存在一些挑战。在这项研究中，我们展示了如何在纳米晶金属有机骨架（nMOF）粒子的保护载体中利用染料分子进行生物成像。具体来说，具有不同分子大小的 Resorufin 和 Rhodamine-6G 分别被封装在 nMOF-801 和 nUiO-67 粒子的紧密配合的孔中。所得的纳米晶颗粒具有高结晶度、均匀的尺寸和形态，并在生物医学环境中保持增强的光致发光性能和异常的稳定性。这些样品进一步用靶向剂功能化，并成功地用于 FL83B（人肝细胞）和 HepG2（人肝癌细胞）的荧光成像，没有细胞毒性。
