Department of Plant Sciences, Faculty of Agronomy "Eliseu Maciel" (FAEM), Federal University of Pelotas, Eliseu Maciel Avenue, Capão do Leão, RS, 96160-000, Brazil.
Department of Botany, Institute of Biology, Federal University of Pelotas, Capão do Leão, RS, Brazil.
Environ Sci Pollut Res Int. 2021 May;28(17):21101-21109. doi: 10.1007/s11356-020-12249-y. Epub 2021 Jan 6.
This study aimed to evaluate the silicon (Si) capacity to attenuate the cadmium (Cd) effects on seed germination and seedling performance of lettuce. The seeds were subjected to three priming levels: without priming, hydropriming, and Si priming. Afterwards, the seeds were placed to germinate on paper moistened with the absence (0 mM) and presence (1 mM) of Cd. Seeds exposed to Cd showed the same percentage of germination verified in seeds unexposed to this metal (99%). Si priming increases 16% the germination speed of seeds not exposed to Cd and promoted greater expression of esterase during seed germination. However, Cd promoted the decrease of the intensity of esterase and acid phosphatase expression, regardless of the seed priming technique used. Although it does not influence the germination percentage of lettuce seeds, Cd markedly reduced the dry weight of seedlings. This harmful effect caused by the Cd was 33% minimized with Si priming. In addition to the lower weight, Cd induced a significant reduction in antioxidant activity in seedlings. However, Si seed priming caused a greater antioxidant activity-with emphasis on catalase-and, consequently, less lipid peroxidation. In conclusion, Si seed priming contributes to minimize the Cd effects in lettuce seedlings.
本研究旨在评估硅（Si）对缓解镉（Cd）对生菜种子萌发和幼苗性能的影响的能力。种子经过三种引发水平处理：无引发、水引发和 Si 引发。然后，将种子放置在湿润的纸上进行萌发，纸的湿润液中含有或不含有 Cd（0 mM 或 1 mM）。暴露于 Cd 的种子与未暴露于该金属的种子（99%）具有相同的萌发百分比。Si 引发增加了未暴露于 Cd 的种子的萌发速度 16%，并在种子萌发过程中促进了酯酶的更高表达。然而，无论使用哪种种子引发技术，Cd 都会降低酯酶和酸性磷酸酶表达的强度。尽管 Si 对种子萌发的百分率没有影响，但 Cd 明显降低了幼苗的干重。这种由 Cd 引起的有害影响，通过 Si 引发处理，可减少 33%。此外，Cd 还会导致幼苗的抗氧化活性显著降低。然而，Si 种子引发处理会引起更大的抗氧化活性——特别强调了过氧化氢酶——因此，脂质过氧化作用减少。总之，Si 种子引发处理有助于减少 Cd 对生菜幼苗的影响。
