Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Freshwater Station, Bangladesh Fisheries Research Institute (BFRI), Mymensingh, 2201, Bangladesh.
Environ Sci Pollut Res Int. 2021 Apr;28(16):20659-20667. doi: 10.1007/s11356-020-11944-0. Epub 2021 Jan 6.
Profenofos is an endocrine-disrupting chemical that can enter into the aquatic ecosystem either through surface runoff or through percolation of a toxicant from the soil. In order to clarify the effect of profenofos on the developmental stages of zebrafish, the embryos were treated with serial dilutions of profenofos (0%, 10%, 25%, and 50% of LC). Embryos were treated with profenofos for 7 days or until hatching. The toxic endpoints assessed include hatching time, survival, malformation, and heartbeats of the embryos. In a 96-h test on zebrafish embryos, the LC of profenofos was 0.057 mg/L. Profenofos considerably lowered survival, increased abnormalities at different ontogenetic stages, and developed malformations of different organs in a concentration-dependent fashion. The identified developmental malformations were fluid accumulation, impaired jaw, short tail, ruptured pectoral and caudal fin, curved body, thin yolk sac tube, and deformed heart. The way of looping arrangement of the heart at the early stage of embryos was significantly influenced by the higher concentration of profenofos. Heartbeat is also reduced significantly in a concentration-dependent fashion. The results show that the zebrafish are susceptible to profenofos even at lower concentrations in the initial stage. Therefore, when used in agricultural areas adjacent to the aquatic environment, endocrine-disrupting chemicals should be used in an appropriate manner.
丙溴磷是一种内分泌干扰化学物质，既可以通过地表径流进入水生态系统，也可以通过土壤中毒物的渗透进入水生态系统。为了阐明丙溴磷对斑马鱼发育阶段的影响，将胚胎用丙溴磷的系列稀释液（LC 的 0%、10%、25%和 50%）处理。胚胎用丙溴磷处理 7 天或直到孵化。评估的毒性终点包括孵化时间、存活率、畸形和胚胎的心跳。在 96 小时的斑马鱼胚胎测试中，丙溴磷的 LC 为 0.057mg/L。丙溴磷显著降低了存活率，增加了不同胚胎发生阶段的异常，并以浓度依赖的方式导致不同器官的畸形。鉴定出的发育畸形包括液体蓄积、下颌畸形、短尾、胸鳍和尾鳍破裂、弯曲的身体、蛋黄囊管变薄和心脏变形。胚胎早期心脏的环曲排列方式受到较高浓度丙溴磷的显著影响。心跳也呈浓度依赖性显著减少。结果表明，即使在较低浓度的初始阶段，斑马鱼也容易受到丙溴磷的影响。因此，当在靠近水生态系统的农业区域使用时，应适当使用内分泌干扰化学物质。
