Khatun Mt Marufa, Mostakim Golam Mohammod, Moniruzzaman Md, Rahman Umme Ohida, Islam M Sadiqul
Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Toxicol Rep. 2021 Feb 23;8:415-421. doi: 10.1016/j.toxrep.2021.02.019. eCollection 2021.
The experiment was explicated to investigate the fenitrothion persuaded genotoxicity in the peripheral erythrocytes of zebrafish () through exposures (10 %, 20 % and 40 % of LC of fenitrothion, i.e., 0.8, 1.6, and 3.2 mg/L, respectively) for variable periods (1, 3, and 7 days) and its subsequent post-exposure recuperation array in pesticide-free water for similar intervals was also evaluated. With the exception of the control group (0% of fenitrothion), the obtained results pointed out that with the promotion of time and concentrations, fenitrothion induced significantly ( < 0.05) higher prevalence and severity of erythrocytic nuclear abnormalities (ENA) such as- notched, micronucleus, nuclear bridges, blebbed, binucleated, nuclear bud and also erythrocytic cellular abnormalities ECA) such as - echinocytic, elongated, tear-drop, crescentic, twin, fusion, and spindle-shaped cells. Recuperation data stated that zebrafish cured spontaneously and aberrated erythrocytic anomalies in all treatments were renormalized according to the concentration and duration dependence. Hence, we concluded that fenitrothion has a dangerous effect on the zebrafish, and this technology can be used to anticipate the sensitivity of aquatic animals to environmental pollution.
本实验旨在研究杀螟硫磷对斑马鱼外周血红细胞的遗传毒性，通过不同浓度（分别为杀螟硫磷LC的10%、20%和40%，即0.8、1.6和3.2mg/L）暴露不同时间（1、3和7天），并评估其在无农药水中类似时间段的暴露后恢复情况。除对照组（0%杀螟硫磷）外，所得结果表明，随着时间和浓度的增加，杀螟硫磷显著（P<0.05）诱导红细胞核异常（ENA）的发生率和严重程度升高，如缺口、微核、核桥、泡状、双核、核芽，以及红细胞细胞异常（ECA），如棘状、拉长、泪滴状、新月状、双细胞、融合和纺锤状细胞。恢复数据表明，斑马鱼能自发恢复，所有处理中异常的红细胞异常均根据浓度和持续时间依赖性恢复正常。因此，我们得出结论，杀螟硫磷对斑马鱼有危险影响，该技术可用于预测水生动物对环境污染的敏感性。
