Wondimu Kokob Teshome, Geletu Abiyot Kelecha
Department of Chemistry, College of Natural and Computational Sciences, Mettu University, Mettu, P.O. Box, 318, Ethiopia.
Heliyon. 2023 Feb 27;9(3):e14121. doi: 10.1016/j.heliyon.2023.e14121. eCollection 2023 Mar.
In this study, analysis of some selected organophosphorus and organochlorine pesticides was carried out in tomato samples collected from the markets of Mettu town, Ilu Aba Bora Zone, Oromia, Ethiopia. The quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method was used for the extraction of collected samples using acetonitrile with 1% acetic acid, anhydrous magnesium sulfate, and anhydrous sodium acetate, followed by dispersive solid-phase extraction (d-SPE). Chromatographic separation and quantitative determination of the pesticides were performed by gas chromatography equipped with a mass spectrometer. The calibration curves for each analyte constructed in tomato samples were linear in the concentration range of 2-200 μg/kg with R > 0.99 and the LODs and LOQs of the method were in the range of 0.01-0.04 μg/kg and 0.04-0.13 μg/kg, respectively. The recovery and precision study results were within acceptable standard guidelines. Of the fourteen pesticides tested, chlordane, hexachlorobenzene, 4, 4'-DDE, 4, 4'-DDD, 4, 4'- DDT, and profenofos were detected at 0.15, 0.09, 0.17, 0.032, 1.24 and 0.062 μg/kg, respectively. Despite the concentrations of the detected pesticides being lower than the maximum residue limit recommended by FAO/WHO, the presence of these toxic chemicals in tomatoes indicates product contamination and requires extensive further investigation with the inclusion of other pesticides.
在本研究中，对从埃塞俄比亚奥罗米亚州伊卢阿巴博拉区梅图镇市场采集的番茄样本中的一些选定有机磷和有机氯农药进行了分析。采用快速、简便、廉价、高效、耐用和安全（QuEChERS）方法，使用含1%乙酸的乙腈、无水硫酸镁和无水醋酸钠对采集的样本进行萃取，随后进行分散固相萃取（d-SPE）。农药的色谱分离和定量测定通过配备质谱仪的气相色谱进行。在番茄样本中构建的各分析物校准曲线在2-200μg/kg浓度范围内呈线性，R>0.99，该方法的检测限和定量限分别在0.01-0.04μg/kg和0.04-0.13μg/kg范围内。回收率和精密度研究结果在可接受的标准指南范围内。在所测试的14种农药中，分别检测到氯丹、六氯苯、4,4'-滴滴伊、4,4'-滴滴滴、4,4'-滴滴涕和丙溴磷的含量为0.15、0.09、0.17、0.032、1.24和0.062μg/kg。尽管检测到的农药浓度低于粮农组织/世界卫生组织建议的最大残留限量，但番茄中存在这些有毒化学物质表明产品受到污染，并需要纳入其他农药进行广泛的进一步调查。
