New York University Abu Dhabi, Abu Dhabi, United Arab Emirates.
Methods Mol Biol. 2021;2229:293-311. doi: 10.1007/978-1-0716-1032-9_14.
One of the fundamental properties of engineered large-scale complex systems is modularity. In synthetic biology, genetic parts exhibit context-dependent behavior. Here, we describe and quantify a major source of such behavior: retroactivity. In particular, we provide a step-by-step guide for characterizing retroactivity to restore the modular description of genetic modules. Additionally, we also discuss how retroactivity can be leveraged to quantify and maximize robustness to perturbations due to interconnection of genetic modules.
工程化的大规模复杂系统的一个基本属性是模块性。在合成生物学中，遗传元件表现出与上下文相关的行为。在这里，我们描述并量化了这种行为的一个主要来源：反作用。具体来说，我们提供了一个逐步的指南，用于描述反作用，以恢复遗传模块的模块化描述。此外，我们还讨论了如何利用反作用来量化和最大化遗传模块互连引起的扰动的鲁棒性。
