1] Centre for Synthetic Biology and Innovation, Imperial College London, London, UK. [2] Department of Bioengineering, Imperial College London, London, UK.
Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6.
Heterologous gene expression can be a significant burden for cells. Here we describe an in vivo monitor that tracks changes in the capacity of Escherichia coli in real time and can be used to assay the burden imposed by synthetic constructs and their parts. We identify construct designs with reduced burden that predictably outperformed less efficient designs, despite having equivalent output.
异源基因表达可能会给细胞带来很大负担。在这里，我们描述了一种体内监测器，它可以实时跟踪大肠杆菌的能力变化，并可用于测定合成构建体及其部分带来的负担。我们发现，尽管输出等效，但具有较低负担的构建体设计可预测性地优于效率较低的设计。
