White E M, Shapiro D L, Allis C D, Gorovsky M A
Department of Biology, University of Rochester, NY 14627.
Nucleic Acids Res. 1988 Jan 11;16(1):179-98. doi: 10.1093/nar/16.1.179.
hv1 is a histone H2A variant found in the transcriptionally active Tetrahymena macronucleus, but not in the transcriptionally inert micronucleus. hv1 also contains antigenic determinants conserved in the histone complements of representatives of all four eukaryotic kingdoms. A cDNA clone encoding hv1 has been isolated and sequenced. Comparison of the derived protein sequence of hv1 with that of the chicken variant H2A.F and the sea urchin variant H2A.F/Z reveals remarkable homology in all but the extreme amino- and carboxy-termini and a small region in the conserved core. Putative regions of conserved antigenicity are discussed. Evidence is presented that suggests that hv1 is a single-copy, intron-containing gene that encodes a polyadenylated message. Unusual features in the 3' flanking sequence and in codon usage are also described. Evidence is also presented showing that hv1 message amounts are ten-fold greater in growing cells than in starved cells.
hv1是一种在转录活跃的草履虫大核中发现的组蛋白H2A变体，但在转录惰性的小核中未发现。hv1还包含在所有四个真核生物界代表的组蛋白补体中保守的抗原决定簇。一个编码hv1的cDNA克隆已被分离和测序。将hv1的推导蛋白质序列与鸡变体H2A.F和海胆变体H2A.F/Z的序列进行比较，结果显示除了极端的氨基和羧基末端以及保守核心中的一个小区域外，其他区域具有显著的同源性。讨论了保守抗原性的推定区域。有证据表明，hv1是一个单拷贝、含内含子的基因，编码一个多聚腺苷酸化的信息。还描述了3'侧翼序列和密码子使用中的异常特征。也有证据表明，hv1信息在生长细胞中的含量比饥饿细胞中高十倍。
