Ball D J, Slaughter C A, Hensley P, Garrard W T
FEBS Lett. 1983 Apr 5;154(1):166-70. doi: 10.1016/0014-5793(83)80896-5.
The minor histone H2A subtype, H2A.Z, has been purified to homogeneity from calf thymus and subjected to automated Edman degradation. The sequence of the first 30 amino acids possesses only 60% homology with major H2A subtypes of the same tissue. This sequence difference is more extreme than that exhibited between evolutionarily distant major H2A subtypes. However, an analysis of secondary structure reveals that H2A.Z and major H2A subtypes exhibit the same general topographical features within their N-terminal domains.
微小组蛋白H2A亚型H2A.Z已从小牛胸腺中纯化至同质，并进行了自动Edman降解。前30个氨基酸的序列与同一组织中的主要H2A亚型仅具有60%的同源性。这种序列差异比进化距离较远的主要H2A亚型之间的差异更为显著。然而，二级结构分析表明，H2A.Z和主要H2A亚型在其N端结构域内表现出相同的总体拓扑特征。
