Firoozi Negar, Kang Yunqing
Department of Ocean & Mechanical Engineering, Florida Atlantic University, 777 Glades Road, Boca Raton, Florida 33431, United States.
Department of Biomedical Science, Florida Atlantic University, 777 Glades Road, Boca Raton, Florida 33431, United States.
ACS Biomater Sci Eng. 2019 Mar 11;5(3):1257-1267. doi: 10.1021/acsbiomaterials.9b00059. Epub 2019 Mar 1.
In spite of the vast research on developing a highly elastic polymer for tissue regeneration, using a renewable resource and a simple, environment-friendly synthesis route to synthesize an elastic polymer has not been successfully achieved yet. The objective of this study was to use a simple melt condensation polymerization method to develop an elastic polymer for tissue regeneration applications. A nature-derived renewable, nontoxic, and inexpensive monomer, xylitol, and a cross-linking agent, dodecanedioic acid, were used to synthesize the new polymer named poly(xylitol-dodecanedioic acid) (PXDDA). Its physicochemical and biological properties were fully characterized. Fourier transform infrared (FTIR) results confirmed the formation of ester bonding in the polymer structure, and thermal analysis results demonstrated that the polymer was completely amorphous. The polymer is highly elastic. Increasing the molar ratio of dodecanedioic acid resulted in lower elasticity, higher hydrophobicity, and lower glass transition temperature. Further, the polymer degradation rate and dye release from the polymer also became slower when the amount of dodecanedioic acid in the composite increased. Biocompatibility studies showed that both the polymeric materials and the degraded products of the polymer did not show any toxicity. Instead, this new polymer significantly promoted cell adhesion and proliferation, compared to a widely used polymer, poly(lactic acid), and tissue culture plates. Interestingly, the PXDDA polymer demonstrated autofluorescent properties. Overall, these results suggest that a new, elastic, biodegradable polymer has been successfully synthesized, and it holds great promise for biomedical applications in drug delivery and tissue engineering.
尽管在开发用于组织再生的高弹性聚合物方面进行了大量研究，但利用可再生资源和简单、环保的合成路线来合成弹性聚合物尚未成功实现。本研究的目的是使用简单的熔融缩聚方法来开发一种用于组织再生应用的弹性聚合物。使用一种天然衍生的可再生、无毒且廉价的单体木糖醇和一种交联剂十二烷二酸来合成名为聚（木糖醇 - 十二烷二酸）（PXDDA）的新型聚合物。对其物理化学和生物学性质进行了全面表征。傅里叶变换红外光谱（FTIR）结果证实了聚合物结构中酯键的形成，热分析结果表明该聚合物完全无定形。该聚合物具有高弹性。增加十二烷二酸的摩尔比会导致弹性降低、疏水性增加和玻璃化转变温度降低。此外，当复合材料中十二烷二酸的量增加时，聚合物的降解速率和染料从聚合物中的释放也会变慢。生物相容性研究表明，聚合物材料及其降解产物均未显示出任何毒性。相反，与广泛使用的聚合物聚乳酸和组织培养板相比，这种新型聚合物显著促进了细胞黏附和增殖。有趣的是，PXDDA聚合物表现出自发荧光特性。总体而言，这些结果表明已成功合成了一种新型的、弹性的、可生物降解的聚合物，它在药物递送和组织工程的生物医学应用中具有巨大潜力。
