Department of Ocean & Mechanical Engineering, Florida Atlantic University, 777 Glades Road, Boca Raton, Florida, 33431, United States.
Department of Biomedical Science, Florida Atlantic University, 777 Glades Road, Boca Raton, Florida, 33431, United States.
Sci Rep. 2020 Jun 26;10(1):10419. doi: 10.1038/s41598-020-67261-6.
Fibroblast growth factor (FGF) plays a vital role in the repair and regeneration of most tissues. However, its low stability, short half-life, and rapid inactivation by enzymes in physiological conditions affect their clinical applications. Therefore, to increase the effectiveness of growth factors and to improve tissue regeneration, we developed an elastic polymeric material poly(xylitol dodecanedioic acid) (PXDDA) and loaded FGF on the PXDDA for sustained drug delivery. In this study, we used a simple dopamine coating method to load FGF on the surface of PXDDA polymeric films. The polydopamine-coated FGF-loaded PXDDA samples were then characterized using FTIR and XRD. The in vitro drug release profile of FGF from PXDDA film and cell growth behavior were measured. Results showed that the polydopamine layer coated on the surface of the PXDDA film enhanced the immobilization of FGF and controlled its sustained release. Human fibroblast cells attachment and proliferation on FGF-immobilized PXDDA films were much higher than the other groups without coatings or FGF loading. Based on our results, the surface modification procedure with immobilizing growth factors shows excellent application potential in tissue regeneration.
成纤维细胞生长因子 (FGF) 在大多数组织的修复和再生中起着至关重要的作用。然而，其在生理条件下的低稳定性、短半衰期和快速被酶失活会影响它们的临床应用。因此，为了提高生长因子的有效性并改善组织再生，我们开发了一种弹性聚合物材料聚（木糖醇十二烷二酸）（PXDDA），并将 FGF 负载在 PXDDA 上用于持续药物输送。在这项研究中，我们使用简单的多巴胺涂层方法将 FGF 负载在 PXDDA 聚合物薄膜的表面上。然后使用傅里叶变换红外光谱 (FTIR) 和 X 射线衍射 (XRD) 对载有 FGF 的 PXDDA 样品进行了表征。测量了 FGF 从 PXDDA 薄膜中的体外药物释放曲线和细胞生长行为。结果表明，多巴胺层涂覆在 PXDDA 薄膜表面上增强了 FGF 的固定化并控制了其持续释放。与其他无涂层或未负载 FGF 的组相比，固定化 FGF 的 PXDDA 薄膜上的人成纤维细胞附着和增殖要高得多。基于我们的结果，表面改性程序与固定生长因子显示出在组织再生中具有优异的应用潜力。
