Davenport Huyer Locke, Zhang Boyang, Korolj Anastasia, Montgomery Miles, Drecun Stasja, Conant Genevieve, Zhao Yimu, Reis Lewis, Radisic Milica
Department of Chemical Engineering and Applied Chemistry, ‡Institute of Biomaterials and Biomedical Engineering, and §Human Biology, University of Toronto, Toronto, Ontario, Canada.
Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, and §Human Biology, University of Toronto, Toronto, Ontario, Canada.
ACS Biomater Sci Eng. 2016 May 9;2(5):780-788. doi: 10.1021/acsbiomaterials.5b00525. Epub 2016 Apr 28.
Polyester biomaterials are used in tissue engineering as scaffolds for implantation of tissues developed in vitro. An ideal biodegradable elastomer for cardiac tissue engineering exhibits a relatively low Young's modulus, with high elongation and tensile strength. Here we describe a novel polyester biomaterial that exhibits improved elastic properties for cardiac tissue engineering applications. We synthesized poly(octamethylene maleate (anhydride) 1,2,4-butanetricarboxylate) (124 polymer) prepolymer gel in a one-step polycondensation reaction. The prepolymer was then molded as desired and exposed to ultraviolet (UV) light to produce a cross-linked elastomer. 124 polymer exhibited highly elastic properties under aqueous conditions that were tunable according to the UV light exposure, monomer composition, and porosity of the cured elastomer. Its elastomeric properties fell within the range of adult heart myocardium, but they could also be optimized for higher elasticity for weaker immature constructs. The polymer showed relatively stable degradation characteristics, both hydrolytically and in a cellular environment, suggesting maintenance of material properties as a scaffold support for potential tissue implants. When assessed for cell interaction, this polymer supported rat cardiac cell attachment in vitro as well as comparable acute in vivo host response when compared to poly(l-lactic acid) control. This suggests the potential applicability of this material as an elastomer for cardiac tissue engineered constructs.
聚酯生物材料在组织工程中用作体外培养组织植入的支架。用于心脏组织工程的理想可生物降解弹性体具有相对较低的杨氏模量、高伸长率和拉伸强度。在此，我们描述了一种新型聚酯生物材料，它在心脏组织工程应用中表现出改善的弹性性能。我们通过一步缩聚反应合成了聚（马来酸（酐）辛亚甲基1,2,4 - 丁三羧酸酯）（124聚合物）预聚物凝胶。然后将预聚物按所需成型并暴露于紫外线（UV）光下以产生交联弹性体。124聚合物在水性条件下表现出高弹性性能，其可根据紫外线暴露、单体组成和固化弹性体的孔隙率进行调节。其弹性性能落在成年心脏心肌的范围内，但也可针对较弱的未成熟构建体进行优化以获得更高的弹性。该聚合物在水解和细胞环境中均表现出相对稳定的降解特性，表明作为潜在组织植入物的支架支撑材料性能得以维持。当评估细胞相互作用时，与聚（L - 乳酸）对照相比，该聚合物在体外支持大鼠心脏细胞附着以及相当的急性体内宿主反应。这表明这种材料作为心脏组织工程构建体弹性体的潜在适用性。
