Yang Shuoshuo, Wang Yong, Wu Xiaoxiao, Sheng Sunren, Wang Tian, Zan Xingjie
School of Ophthalmology and Optometry, Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, PR China.
Wenzhou Institute of Biomaterials and Engineering, CNITECH, Chinese Academy of Sciences, Wenzhou, Zhejiang Province 325001, PR China.
ACS Biomater Sci Eng. 2019 Jul 8;5(7):3582-3594. doi: 10.1021/acsbiomaterials.9b00717. Epub 2019 Jun 25.
A multifunctional (TA/Lys) film, featuring good antioxidant property, fast cell attachment at the initial stage, enhanced osteogenesis, and broad-spectrum antibacterial property, was constructed by the layer-by-layer (LBL) method. The building process was monitored by quartz crystal microbalance with dissipation (QCM-D); the physical properties, such as topography, stiffness in dry and liquid state, and conformation of Lys in the film, were thoroughly characterized. These physical properties were modulated by varying the salt concentration at which the film was constructed. The film not only allows for favorable cell attachment and proliferation of preosteoblasts Mc3t3-E1 but also provides antibacterial property against Gram-positive bacteria, and , and Gram-negative bacteria, . It also displays good antioxidant property, which plays a critical role on fast cell attachment at the initial stage.
通过层层（LBL）法构建了一种多功能（TA/Lys）膜，其具有良好的抗氧化性能、初始阶段快速的细胞附着、增强的成骨作用和广谱抗菌性能。通过具有耗散功能的石英晶体微天平（QCM-D）监测构建过程；对膜的物理性质，如形貌、干态和液态下的刚度以及膜中Lys的构象进行了全面表征。通过改变构建膜时的盐浓度来调节这些物理性质。该膜不仅有利于前成骨细胞Mc3t3-E1的细胞附着和增殖，还具有针对革兰氏阳性菌和革兰氏阴性菌的抗菌性能。它还显示出良好的抗氧化性能，这在初始阶段对快速细胞附着起着关键作用。
