Li Huaqiong, Gao Chenyuan, Tang Lin, Wang Chenou, Chen Qiong, Zheng Qianyi, Yang Shuoshuo, Sheng Sunren, Zan Xingjie
School of Ophthalmology and Optometry, Eye Hospital, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang Province 325035, P.R. China.
Wenzhou Institute, University of Chinese Academy of Sciences (Wenzhou Institute of Biomaterials & Engineering), 16 Xinsan Road, Wenzhou 325001, P.R. China.
ACS Appl Bio Mater. 2020 Jan 21;3(1):673-684. doi: 10.1021/acsabm.9b01017. Epub 2020 Jan 8.
Dental implants have great potential in the global market, around $3.7 billion in 2015, which will increase to $7 billion in 2023 with an annual increase rate of 8.2%. Incorporating antibacterial and osteogenic agents into implants is helpful to make the dental implants successful, which can be endowed by coatings. In recent years, graphene oxide (GO) and its composite materials have shown advances in the biomedical field. Lysozyme (Lys) and tannic acid (TA) are naturally derived, with promising antibacterial and osteogenic properties as well. In the present study, the strong antibacterial and enhanced osteogenic multilayer coating is fabricated using the facile and controllable layer by layer (LBL) technique to integrate GO, Lys, and TA. The thickness of coating exhibited a continuous growth with the deposited process as proved from UV-vis and ellipsometry data, and the physical properties of the coating, such as wettability, roughness, and stiffness are well characterized. The coatings exhibited the synergic effect on the killing bacteria, both Gram-negative bacteria and Gram-positive bacteria represented by and , respectively, and enhancing osteogenesis of dental pulp stem cells (hDPSCs), showing the potential application on coatings of dental implants. Thus, the strategy applied here will inspire the design and development of dual functional surfaces for the success of implanted dental surface in future.
牙科植入物在全球市场具有巨大潜力，2015年约为37亿美元，到2023年将增至70亿美元，年增长率为8.2%。将抗菌和成骨剂融入植入物有助于使牙科植入物获得成功，这可以通过涂层来实现。近年来，氧化石墨烯（GO）及其复合材料在生物医学领域已展现出进展。溶菌酶（Lys）和单宁酸（TA）也是天然来源的，同样具有良好的抗菌和成骨特性。在本研究中，采用简便可控的层层（LBL）技术制备了具有强抗菌和增强成骨性能的多层涂层，以整合GO、Lys和TA。紫外可见光谱和椭偏仪数据证明，涂层厚度随沉积过程持续增长，且涂层的物理性质，如润湿性、粗糙度和硬度等也得到了很好的表征。该涂层对杀灭细菌具有协同作用，分别以革兰氏阴性菌和革兰氏阳性菌为代表，同时还能增强牙髓干细胞（hDPSCs）的成骨能力，显示出在牙科植入物涂层方面的潜在应用价值。因此，这里应用的策略将为未来成功植入牙科表面的双功能表面的设计和开发提供启发。
