Wang Guofeng, Zhu Yaxin, Zan Xingjie, Li Meng
The Fourth Affiliated Hospital of China Medical University, Shenyang, China.
Oujiang Laboratory, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, China.
Front Bioeng Biotechnol. 2021 Jul 19;9:718255. doi: 10.3389/fbioe.2021.718255. eCollection 2021.
There is a substantial global market for orthopedic implants, but these implants still face the problem of a high failure rate in the short and long term after implantation due to the complex physiological conditions in the body. The use of multifunctional coatings on orthopedic implants has been proposed as an effective way to overcome a range of difficulties. Here, a multifunctional (TA@HA/Lys) coating composed of tannic acid (TA), hydroxyapatite (HA), and lysozyme (Lys) was fabricated in a layer-by-layer (LBL) manner, where TA deposited onto HA firmly stuck Lys and HA together. The deposition of TA onto HA, the growth of (TA@HA/Lys), and multiple related biofunctionalities were thoroughly investigated. Our data demonstrated that such a hybrid coating displayed antibacterial and antioxidant effects, and also facilitated the rapid attachment of cells [both mouse embryo osteoblast precursor cells (MC3T3-E1) and dental pulp stem cells (DPSCs)] in the early stage and their proliferation over a long period. This accelerated osteogenesis and promoted bone formation . We believe that our findings and the developed strategy here could pave the way for multifunctional coatings not only on orthopedic implants, but also for additional applications in catalysts, sensors, tissue engineering, etc.
全球对骨科植入物有巨大的市场需求，但由于体内复杂的生理条件，这些植入物在植入后的短期和长期仍面临高失败率的问题。在骨科植入物上使用多功能涂层已被提议作为克服一系列困难的有效方法。在此，通过层层（LBL）方式制备了一种由单宁酸（TA）、羟基磷灰石（HA）和溶菌酶（Lys）组成的多功能（TA@HA/Lys）涂层，其中沉积在HA上的TA将Lys和HA牢固地结合在一起。深入研究了TA在HA上的沉积、（TA@HA/Lys）的生长以及多种相关生物功能。我们的数据表明，这种复合涂层具有抗菌和抗氧化作用，并且在早期促进了细胞[小鼠胚胎成骨细胞前体细胞（MC3T3-E1）和牙髓干细胞（DPSC）]的快速附着及其长期增殖。这加速了成骨过程并促进了骨形成。我们相信，我们的研究结果和在此开发的策略不仅可以为骨科植入物上的多功能涂层铺平道路，也可为催化剂、传感器、组织工程等其他应用开辟道路。
