Shi Haishan, Ye Xiaoling, Zhang Jing, Ye Jiandong
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China.
National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, China.
ACS Biomater Sci Eng. 2019 Jan 14;5(1):262-271. doi: 10.1021/acsbiomaterials.8b00871. Epub 2018 Dec 3.
Toward repairing critical-sized bone defects, calcium phosphate cement (CPC) has been well recognized as a fairly promising bone graft because of its properties of injectability, self-setting, biocompatibility, and osteoconductivity. However, poor osteogenic capacity of CPC still limits its applications for meeting the demands of bone healing. In this work, chondroitin sulfate (CS), as an important component of the extracellular matrix network, was introduced into CPC to enhance its osteogenesis ability. Incorporation of CS had no evident effect on the phase, morphology, apparent porosity, and compressive strength of hydrated cement products, but it notably enhanced the injectability and improved the antiwashout property of the cement pastes. CS was able to be sustainably released from CS-CPCs in a CS-dose-dependent manner and supposed to have a long-term release potential for constant biological stimulation. CS-CPCs markedly accelerated the preferential adsorption of fibronectin. Furthermore, CS-CPCs significantly improved the adhesion, proliferation, and osteogenic differentiation of bone mesenchymal stem cells, which was synergistically mediated by the adhesion events of cells on the hydrated cements and the stimulation effects of CS molecules. Herein, utilization of CS is supposed to endow injectable calcium phosphate bone cements with enhanced osteogenic capacity and suitable physicochemical properties for numerous promising orthopedic applications.
为修复临界尺寸骨缺损，磷酸钙骨水泥（CPC）因其可注射性、自固化性、生物相容性和骨传导性等特性，已被公认为是一种颇具前景的骨移植材料。然而，CPC较差的成骨能力仍限制了其在满足骨愈合需求方面的应用。在本研究中，硫酸软骨素（CS）作为细胞外基质网络的重要组成部分，被引入到CPC中以增强其成骨能力。CS的掺入对水合水泥产品的相、形态、表观孔隙率和抗压强度没有明显影响，但显著提高了可注射性并改善了水泥浆体的抗冲刷性能。CS能够以CS剂量依赖的方式从CS-CPC中持续释放，并且具有长期释放以持续进行生物刺激的潜力。CS-CPC显著加速了纤连蛋白的优先吸附。此外，CS-CPC显著改善了骨间充质干细胞的黏附、增殖和成骨分化，这是由细胞在水合水泥上的黏附事件和CS分子的刺激作用协同介导的。在此，CS的应用有望赋予可注射磷酸钙骨水泥增强的成骨能力和适合众多有前景的骨科应用的理化性质。
