Raja Farah N S, Worthington T, Isaacs Mark A, Forto Chungong Louis, Burke Bernard, Addison Owen, Martin Richard A
School of Life & Health Science and Aston Research Centre for Healthy Ageing, Aston University, Aston Triangle, Birmingham, B4 7ET, United Kingdom.
Department of Chemistry, University College London, 20 Gordon Street, Kings Cross, London, WC1H 0AJ, United Kingdom.
ACS Biomater Sci Eng. 2019 Jan 14;5(1):283-293. doi: 10.1021/acsbiomaterials.8b01045. Epub 2018 Dec 4.
Bioactive phosphate glasses are of considerable interest for a range of soft and hard tissue engineering applications. The glasses are degradable and can release biologically important ions in a controlled manner. The glasses can also potentially be used as an antimicrobial delivery system. In the given study, novel cobalt-doped phosphate-based glasses, (PO)(NaO)(CaO)(CoO) where 0 ≤ (mol %) ≤ 10, were manufactured and characterized. As the cobalt oxide concentration increased, the rate of dissolution was observed to decrease. The antimicrobial potential of the glasses was studied using direct and indirect contact methods against both (NCTC 10538) (ATCC 6538) and (ATCC 76615). The results showed strong, time dependent, and strain specific antimicrobial activity of the glasses against microorganisms when in direct contact. Antimicrobial activity () ≥ 2 was observed within 2 h against , whereas a similar effect was achieved in 6 h against and . However, when in indirect contact, the dissolution products from the bioactive glasses failed to show an antimicrobial effect. Following direct exposure to the glasses for 7 days, osteoblast-like SAOS-2 cells showed a 5-fold increase in VEGF mRNA while THP-1 monocytic cells showed a 4-fold increase in VEGF mRNA expression when exposed to 10% CoO-doped glass compared with the cobalt free control glass. Endothelial cells stimulated with conditioned medium taken from cell cultures of THP-1 monocytes exposed to 10% CoO doped glass showed clear tubelike structure (blood vessel) formation after 4 h.
生物活性磷酸盐玻璃在一系列软组织和硬组织工程应用中具有相当大的吸引力。这些玻璃是可降解的，并且能够以可控的方式释放具有生物学重要性的离子。这些玻璃还可能用作抗菌递送系统。在给定的研究中，制备并表征了新型钴掺杂的磷酸盐基玻璃，即(PO)(NaO)(CaO)(CoO)，其中0 ≤ (摩尔百分比) ≤ 10。随着氧化钴浓度的增加，观察到溶解速率降低。使用直接和间接接触方法研究了这些玻璃对金黄色葡萄球菌（NCTC 10538）、表皮葡萄球菌（ATCC 6538）和铜绿假单胞菌（ATCC 76615）的抗菌潜力。结果表明，当直接接触时，这些玻璃对微生物具有强烈的、时间依赖性的和菌株特异性的抗菌活性。在2小时内对金黄色葡萄球菌观察到抗菌活性（）≥ 2，而对表皮葡萄球菌和铜绿假单胞菌在6小时内达到类似效果。然而，当间接接触时，生物活性玻璃的溶解产物未显示出抗菌效果。在直接暴露于这些玻璃7天后，与无钴对照玻璃相比，成骨样SAOS - 2细胞在暴露于10% CoO掺杂玻璃时VEGF mRNA增加了5倍，而THP - 1单核细胞在暴露于10% CoO掺杂玻璃时VEGF mRNA表达增加了4倍。用来自暴露于10% CoO掺杂玻璃的THP - 1单核细胞的细胞培养条件培养基刺激的内皮细胞在4小时后显示出清晰的管状结构（血管）形成。
