Kuhn J
Antimicrob Agents Chemother. 1977 Sep;12(3):322-7. doi: 10.1128/AAC.12.3.322.
Simple and rapid techniques were developed that allow detection of the effects of a wide range of tryptophan analogs in Escherichia coli. (i) By using certain supersensitive mutant strains, analogs that were without effect on a wild-type strain were shown to be inhibitory. (ii) Many other analogs could inhibit the utilization of d-tryptophan when present as l-tryptophan replacements in a trp dadR strain. (iii) Another approach was to test the ability of a given analog to reverse the inhibition caused by an inhibitory analog. These combined approaches revealed activities in 26 analogs out of a total of 40 that were inactive by testing solely on a wild-type strain. The route of entry of inhibitory analogs was determined unambiguously by comparing their effect on aroP(+) (aromatic permease) and aroP strains. Uptake studies were also performed to determine whether various analogs compete for entry via the aromatic permease system. Many tryptophan analogs enter the cell via this system. The methods developed here should have general applicability to the testing of analogs of a variety of other metabolites.
开发了简单快速的技术，可用于检测多种色氨酸类似物对大肠杆菌的影响。(i) 通过使用某些超敏感突变菌株，已证明对野生型菌株无影响的类似物具有抑制作用。(ii) 当许多其他类似物作为色氨酸替代物存在于 trp dadR 菌株中时，它们可以抑制 d-色氨酸的利用。(iii) 另一种方法是测试给定类似物逆转抑制性类似物引起的抑制作用的能力。这些综合方法揭示了总共 40 种类似物中有 26 种具有活性，而仅在野生型菌株上进行测试时这些类似物是无活性的。通过比较抑制性类似物对 aroP(+)（芳香族通透酶）和 aroP 菌株的影响，明确确定了抑制性类似物的进入途径。还进行了摄取研究，以确定各种类似物是否通过芳香族通透酶系统竞争进入细胞。许多色氨酸类似物通过该系统进入细胞。这里开发的方法应该普遍适用于测试多种其他代谢物的类似物。
