大肠杆菌低亲和力色氨酸特异性通透酶基因的定位
Location of the gene for the low-affinity tryptophan-specific permease of Escherichia coli.
作者信息
Edwards R M, Yudkin M D
出版信息
Biochem J. 1982 May 15;204(2):617-9. doi: 10.1042/bj2040617.
Abstract
L-Tryptophan uptake was assayed under conditions in which the aroT gene had been inactivated by deletion and the product of the aroP permease was competitively inhibition. A mutant carrying a deletion from bgl through tnaA showed negligible L-tryptophan uptake, in contrast to a strain possessing an intact tna region or to strains carrying point mutations in tna. The ability to take up L-tryptophan was not restored by lysogenizing the tna-deleted strain with lambda tna+.
摘要
在aroT基因通过缺失而失活且aroP通透酶产物受到竞争性抑制的条件下测定L-色氨酸摄取。与具有完整tna区域的菌株或tna中携带点突变的菌株相比，携带从bgl到tnaA缺失的突变体显示出可忽略不计的L-色氨酸摄取。用λtna+对tna缺失菌株进行溶原化处理并不能恢复其摄取L-色氨酸的能力。
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