Sneller M R, Hariri A, Sorenson W G, Larsh H W
Antimicrob Agents Chemother. 1977 Sep;12(3):390-4. doi: 10.1128/AAC.12.3.390.
Trichothecin (T-cin), amphotericin B (AB), and 5-fluorocytosine (FC) were compared singly and in combination for capacities to inhibit growth of Cryptococcus neoformans in culture and to protect mice bearing infections with this yeast. The minimum inhibitory concentrations for T-cin, AB, and FC were found to be 0.5, 0.2, and 5.0 mug/ml, respectively. In vitro viability studies demonstrated a marked reduction in colony counts with the AB-FC combination and additive effects with the AB-T-cin and FC-T-cin combinations for a 3-day period. In mice infected intravenously with C. neoformans, the mean effective dose for AB was 0.38 mg/kg, and for FC it was 100 mg/kg for a 30-day treatment period. No mean effective dose could be ascertained when T-cin was tested at doses of 0.1 to 50 mg/kg. Despite this, marked beneficial effects were noted in vivo with the AB-T-cin combination, whereas additive effects and indifference were observed for AB-FC and FC-T-cin combinations, respectively. High-dose T-cin controls survived despite having received a cumulative dosage of more than twice the reported (LD(50)) mean lethal dose value.
对单端孢霉烯族毒素（T - 毒素）、两性霉素B（AB）和5 - 氟胞嘧啶（FC）分别及联合使用时抑制新型隐球菌在培养基中生长以及保护感染该酵母菌的小鼠的能力进行了比较。发现T - 毒素、AB和FC的最低抑菌浓度分别为0.5、0.2和5.0微克/毫升。体外生存能力研究表明，AB - FC联合使用时菌落计数显著减少，AB - T - 毒素和FC - T - 毒素联合使用在3天内有相加作用。在静脉注射新型隐球菌感染的小鼠中，30天治疗期内AB的平均有效剂量为0.38毫克/千克，FC为100毫克/千克。当以0.1至50毫克/千克的剂量测试T - 毒素时，无法确定其平均有效剂量。尽管如此，AB - T - 毒素联合使用在体内有显著的有益效果，而AB - FC和FC - T - 毒素联合使用分别观察到相加作用和无差异作用。高剂量T - 毒素对照组尽管接受了累计剂量超过报告的（半数致死量）平均致死剂量值两倍的剂量，但仍存活下来。
