Zhang Yu, Ding Yifeng, Li Wanning, Zhu Wenjuan, Wang Jia, Wang Xiaohong
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Pathogens. 2021 Jan 4;10(1):34. doi: 10.3390/pathogens10010034.
is a globally distributed zoonotic pathogen. Among them, . Pullorum is a host-specific pathogen that seriously affects the development of the poultry breeding industry in China. It mainly infects chickens and can cause white scabs, and the mortality rate after infection is almost 100%. As antibiotics are widely used in animal feed and other production processes, resistance has gradually increased. Therefore, there is an increasing need to develop new technologies to control multi-drug resistant (MDR) pathogens and confirm their actual effectiveness in the target food matrix. Bacteriophage can efficiently and specifically lyse bacteria, and will be a potential bactericide to replace antibiotics. In this study, 34 strains of bacteriophages were isolated from environmental resources. Therein, phage Pu20 with the widest host spectrum had the strongest ability to lyse tested strains. Further studies showed that Pu20 had high pH tolerance and heat resistance, short incubation period. Pu20 can effectively inhibit the growth of two strains of MDR in liquid egg white and yolk at 4 ℃ and 25 ℃, respectively. According to morphological and phylogenetic analysis, Pu20 belongs to the Podoviridae family. Genomic analysis of Pu20 indicates a linear 59435 bp dsDNA sequence with no homology to virulence or antibiotic resistance-related genes. Together, these results sheds light on the potential biocontrol application value of Pu20 in food products.
是一种全球分布的人畜共患病原体。其中，鸡白痢沙门氏菌是一种宿主特异性病原体，严重影响中国家禽养殖业的发展。它主要感染鸡，可引起白色结痂，感染后的死亡率几乎为100%。由于抗生素广泛应用于动物饲料和其他生产过程，其耐药性逐渐增加。因此，越来越需要开发新技术来控制多重耐药（MDR）病原体，并确认它们在目标食品基质中的实际效果。噬菌体可以高效且特异性地裂解细菌，将成为替代抗生素的潜在杀菌剂。在本研究中，从环境资源中分离出34株噬菌体。其中，宿主谱最广的噬菌体Pu20裂解受试菌株的能力最强。进一步研究表明，Pu20具有高pH耐受性和耐热性，潜伏期短。Pu20分别在4℃和25℃下能有效抑制蛋清和蛋黄中两株多重耐药鸡白痢沙门氏菌的生长。根据形态学和系统发育分析，Pu20属于短尾噬菌体科。Pu20的基因组分析表明其为线性59435 bp双链DNA序列，与毒力或抗生素抗性相关基因无同源性。综上所述，这些结果揭示了Pu20在食品中的潜在生物防治应用价值。
