Song Yaxiong, Gu Wentao, Hu Yaozhong, Zhang Bowei, Wang Jin, Sun Yi, Fu Wenhui, Li Xinyang, Xing Xiaolong, Wang Shuo
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.
Foods. 2024 Sep 28;13(19):3103. doi: 10.3390/foods13193103.
Foodborne pathogens, such as , are major factors that pose significant threats to global food safety and public health. is a prominent serotype contributing to non-typhoidal salmonellosis, which is a prevalent foodborne illness affecting humans and animals. Bacteriophages are considered one of the most environmentally friendly biocontrol agents, particularly in the food industry, owing to their high specificity and high safety. However, the emergency of phage-resistant mutants limits the biocontrol effect of phage treatment, leading to the requirement for a high diversity of lytic phages. Therefore, the study isolated and characterized two novel lytic bacteriophages (SPYS_1 and SPYS_2) targeting ATCC14028 and evaluated their effectiveness in reducing the contamination rates for milk and chicken tenders. Morphological and genomic analyses indicated that phages SPYS_1 and SPYS_2 are novel species classified under the and the , respectively. Both phages exhibited high stability across a broad range of thermal and pH conditions. The one-step growth curve result suggested that both phages had a short adsorption time and a large burst size in a single lytic cycle. The phage SPYS_1 demonstrated a noteworthy inhibition effect on the growth of ATCC14028 in milk, resulting in a ~2-log reduction within the 2 to 4 h range. Overall, both phages have shown significant potential for application in food safety in the future.
食源性病原体，如 ，是对全球食品安全和公众健康构成重大威胁的主要因素。 是导致非伤寒沙门氏菌病的一种突出血清型，非伤寒沙门氏菌病是一种影响人类和动物的常见食源性疾病。噬菌体因其高度特异性和高安全性，被认为是最环保的生物防治剂之一，尤其是在食品工业中。然而，噬菌体抗性突变体的出现限制了噬菌体处理的生物防治效果，导致需要高度多样化的裂解性噬菌体。因此，该研究分离并鉴定了两种针对 ATCC14028的新型裂解性噬菌体（SPYS_1和SPYS_2），并评估了它们在降低牛奶和鸡胸肉污染率方面的有效性。形态学和基因组分析表明，噬菌体SPYS_1和SPYS_2分别是分类在 和 下的新物种。两种噬菌体在广泛的温度和pH条件下均表现出高稳定性。一步生长曲线结果表明，两种噬菌体在单个裂解周期中均具有短吸附时间和大爆发量。噬菌体SPYS_1对牛奶中 ATCC14028的生长表现出显著的抑制作用，在2至4小时内导致约2个对数级的减少。总体而言，两种噬菌体在未来食品安全应用中均显示出巨大潜力。
