Liu Yifan, Liu Zhengqi, Gao Ya, Gao Weiwei, Hou Zhaosheng, Zhu Yuzheng
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China.
Sagacis Biotechnology Co., Ltd., Jinan 250101, China.
Membranes (Basel). 2021 Jan 4;11(1):37. doi: 10.3390/membranes11010037.
In the paper, the chitooligosaccharide (CHO) was surface-grafted on the medical segmented poly(ester-urethane) (SPU) film by a facile two-step procedure to improve the surface biocompatibility. By chemical treatment of SPU film with hexamethylene diisocyanate under mild reaction condition, free -NCO groups were first introduced on the surface with high grafting density, which were then coupled with -NH groups of CHO to immobilize CHO on the SPU surface (SPU-CHO). The CHO-covered surface was characterized by FT-IR and water contact angle test. Due to the hydrophilicity of CHO, the SPU-CHO possessed higher surface hydrophilicity and faster hydrolytic degradation rate than blank SPU. The almost overlapping stress-strain curves of SPU and SPU-CHO films demonstrated that the chemical treatments had little destruction on the intrinsic properties of the substrate. In addition, the significant inhibition of platelet adhesion and protein adsorption on CHO-covered surface endowed SPU-CHO an outstanding surface biocompatibility (especially blood compatibility). These results indicated that the CHO-grafted SPU was a promising candidate as blood-contacting biomaterial for biomedical applications.
在该论文中，通过一种简便的两步法将壳寡糖（CHO）表面接枝到医用嵌段聚（酯 - 聚氨酯）（SPU）薄膜上，以改善其表面生物相容性。在温和的反应条件下，用六亚甲基二异氰酸酯对SPU薄膜进行化学处理，首先在表面引入具有高接枝密度的游离 -NCO 基团，然后将这些基团与CHO的 -NH 基团偶联，从而将CHO固定在SPU表面（SPU - CHO）。通过傅里叶变换红外光谱（FT - IR）和水接触角测试对覆盖有CHO的表面进行了表征。由于CHO的亲水性，SPU - CHO比空白SPU具有更高的表面亲水性和更快的水解降解速率。SPU和SPU - CHO薄膜几乎重叠的应力 - 应变曲线表明，化学处理对基材的固有性能几乎没有破坏。此外，在覆盖有CHO的表面上血小板粘附和蛋白质吸附受到显著抑制，赋予了SPU - CHO出色的表面生物相容性（尤其是血液相容性）。这些结果表明，接枝了CHO的SPU作为用于生物医学应用的血液接触生物材料是一个有前途的候选者。
