Tweeten K A, Bulla L A, Consigli R A
Appl Environ Microbiol. 1977 Sep;34(3):320-7. doi: 10.1128/aem.34.3.320-327.1977.
A procedure was developed for purification of a granulosis virus inclusion body produced in vivo in the Indian meal moth, Plodia interpunctella (Hübner). Purification was accomplished by differential centrifugation, treatment with sodium deoxycholate, and velocity sedimentation in sucrose gradients. The adequacy of the procedure was confirmed by mixing experiments in which uninfected, radioactively labeled larvae were mixed with infected, unlabeled larvae. After purification, the virus was shown to be free of host tissue, to retain its physical integrity, and to be highly infectious per os. Preparations of purified virus consisted of homogeneous populations of intact inclusion bodies (210 by 380 nm) whose buoyant density was 1.271 g/cm3 when centrifuged to equilibrium in sucrose gradients. Electron microscopy of thin-sectioned virus or of virus sequentially disrupted on electron microscope grids demonstrated three components: protein matrix, envelope, and nucleocapsid.
已开发出一种用于纯化在印度谷螟（Plodia interpunctella (Hübner)）体内产生的颗粒体病毒包涵体的方法。纯化通过差速离心、脱氧胆酸钠处理以及在蔗糖梯度中的速度沉降来完成。通过将未感染的、放射性标记的幼虫与感染的、未标记的幼虫混合的混合实验，证实了该方法的充分性。纯化后，病毒被证明不含宿主组织，保持其物理完整性，并且经口具有高度传染性。纯化病毒制剂由完整包涵体（210×380纳米）的均匀群体组成，当在蔗糖梯度中离心至平衡时，其浮力密度为1.271克/立方厘米。对超薄切片病毒或在电子显微镜网格上依次破坏的病毒进行电子显微镜观察，显示出三种成分：蛋白质基质、包膜和核衣壳。
