Rohrmann G F, Martignoni M E, Beaudreau G S
Appl Environ Microbiol. 1978 Apr;35(4):690-3. doi: 10.1128/aem.35.4.690-693.1978.
A reliable method was developed for the quantitative determination of two nuclear polyhcdrosis viruses present in commercially prepared viral insecticides used against Orgyia pseudotsugata. Deoxyribonucleic acids, from nuclear polyhedrosis bundle virus and nuclear polyhedrosis single-rod virus, were separated on CsCl gradients according to their respective buoyant densities, 1.715 and 1.704 g/ml. The proportions of the two viruses were quantified by measuring the relative absorbance at 254 nm of their deoxyribonucleic acid peaks.
开发了一种可靠的方法，用于定量测定商业制备的用于防治伪毒蛾的病毒杀虫剂中存在的两种核型多角体病毒。核型多角体束状病毒和核型多角体单杆状病毒的脱氧核糖核酸，根据它们各自的浮力密度1.715和1.704 g/ml，在氯化铯梯度上进行分离。通过测量其脱氧核糖核酸峰在254 nm处的相对吸光度来定量两种病毒的比例。
