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高脂饮食和运动对生长发育期大鼠睾丸中KISS-1/GPR54表达的影响。

The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats.

作者信息

Feng Junpeng, Xu Rui, Li Yafei, Zhou Qishu, Song Ge, Deng Yimin, Yan Yi

机构信息

Beijing Sports University, Beijing, 100084, China.

Nanjing Sport Institute, Nanjing, 210014, China.

出版信息

Nutr Metab (Lond). 2021 Jan 6;18(1):1. doi: 10.1186/s12986-020-00517-0.

DOI:10.1186/s12986-020-00517-0
PMID:33407658
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7788936/
Abstract

PURPOSE

To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th.

METHOD

128 three-week-old weaned rats underwent high-fat diet and exercise (60-70% VO, 1 h/day, 5 days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sample time points were set on the PND 21st, 35th, 43rd, and 56th. The testicular testosterone and the mRNA content, and protein content of KISS-1 and GPR54 in testis tissue were detected by ELISA, RT-qPCR, and Western blotting.

RESULT

(1) The protein of KISS-1 and GPR54 increased gradually during the growing period. KISS-1 mRNA peaked at 35D and GPR54 peaked at 43D. (2) High-fat diet affected the expression of the KISS-1/GPR54 system in rat testis and reduced the expression level of KISS-1 protein. (3) 60-70% VOmax exercise decreased the KISS-1/GPR54 expression level. Exercise intervention improved testicular development in rats with a high-fat diet.

CONCLUSION

The expression of KISS-1/GPR54 increased during the growing period. High-fat diet can downregulate the protein and gene expression of KISS-1/GPR54 and change the expression trend. 60-70% VOmax exercise decreased the expression of KISS-1/GPR54, which may be involved in the effects of exercise on high-fat dietary sex hormone disorders.

摘要

目的

研究出生后第21天至56天大鼠睾丸中KISS-1和G蛋白偶联受体54的表达情况。

方法

128只三周龄断奶大鼠接受高脂饮食和运动（60-70%最大摄氧量，每天1小时，每周5天）干预，并随机分为C组、CE组、HC组或HE组。在出生后第21天、35天、43天和56天设置样本时间点。采用酶联免疫吸附测定法（ELISA）、逆转录定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹法检测睾丸组织中的睾酮、KISS-1和GPR54的mRNA含量及蛋白质含量。

结果

（1）KISS-1和GPR54蛋白在生长期间逐渐增加。KISS-1 mRNA在35日龄时达到峰值，GPR54 mRNA在43日龄时达到峰值。（2）高脂饮食影响大鼠睾丸中KISS-1/GPR54系统的表达，降低KISS-1蛋白的表达水平。（3）60-70%最大摄氧量运动降低了KISS-1/GPR54的表达水平。运动干预改善了高脂饮食大鼠的睾丸发育。

结论

KISS-1/GPR54的表达在生长期间增加。高脂饮食可下调KISS-1/GPR54的蛋白质和基因表达并改变表达趋势。60-70%最大摄氧量运动降低了KISS-1/GPR54的表达，这可能参与了运动对高脂饮食性激素紊乱的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/89c83eaba159/12986_2020_517_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/480c710981ce/12986_2020_517_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/3e30e0c68a7a/12986_2020_517_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/f286837777e1/12986_2020_517_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/c91ee2ec6100/12986_2020_517_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/f4f41550c65e/12986_2020_517_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/9bd92d319d51/12986_2020_517_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/89c83eaba159/12986_2020_517_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/480c710981ce/12986_2020_517_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/3e30e0c68a7a/12986_2020_517_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/f286837777e1/12986_2020_517_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/c91ee2ec6100/12986_2020_517_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/f4f41550c65e/12986_2020_517_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/9bd92d319d51/12986_2020_517_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/73ca/7788936/89c83eaba159/12986_2020_517_Fig7_HTML.jpg

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