Kim Ae Ri, Lim Yun Kyong, Kook Joong-Ki, Bak Eun-Jung, Yoo Yun-Jung
Department of Oral Biology, College of Dentistry, Yonsei University, Seoul, Republic of Korea.
Department of Applied Life Science, The Graduate School, Yonsei University, Seoul, Republic of Korea.
Lab Anim Res. 2021 Jan 6;37(1):5. doi: 10.1186/s42826-020-00080-y.
Increases of neutrophils and osteoclasts are pathological changes of periodontitis. RANKL is an osteoclast differentiation factor. The effect of periodontopathogen LPS on RANKL-expressing neutrophils has not been clarified yet. We evaluated numerical changes of RANKL-expressing neutrophils in air pouches of mice injected with LPSs of Fusobacterium nucleatum and Porphyromonas gingivalis. Mice with air pouches were assigned into saline (C)-, E. coli LPS- (Ec LPS)-, F. nucleatum LPS (Fn LPS)-, P. gingivalis LPS (Pg LPS)-, and Fn LPS and Pg LPS (Fn + Pg LPS)-injected groups. CD11bLy6G neutrophils and CD11bLy6GRANKL neutrophils in blood and air pouch exudates were determined by flow cytometry. In blood, compared to the C group, the Fn LPS group showed increases of CD11bLy6G neutrophils and CD11bLy6GRANKL neutrophils whereas the Pg LPS group showed no significant differences. These increases in the Fn LPS group were not different to those in the Ec LPS group. In exudates, Fn LPS and Pg LPS groups showed increases of CD11bLy6G neutrophils and CD11bLy6GRANKL neutrophils compared to the C group. Increased levels in the Fn LPS group were not different to those in the Ec LPS group, but Pg LPS group was lower than those in the Ec LPS group. In blood and exudates, the Fn + Pg LPS group showed no difference in levels of these neutrophils compared to the Ec LPS group. LPSs of F. nucleatum and P. gingivalis increased RANKL-expressing neutrophils although the degrees of increases were different. These suggest that periodontopathogen LPS can act as a stimulant to increase RANKL-expressing neutrophils.
中性粒细胞和破骨细胞增多是牙周炎的病理变化。核因子κB受体活化因子配体（RANKL）是一种破骨细胞分化因子。牙周病原体脂多糖（LPS）对表达RANKL的中性粒细胞的影响尚未阐明。我们评估了注射具核梭杆菌和牙龈卟啉单胞菌LPS的小鼠气袋中表达RANKL的中性粒细胞的数量变化。将有气袋的小鼠分为注射生理盐水（C）、大肠杆菌LPS（Ec LPS）、具核梭杆菌LPS（Fn LPS）、牙龈卟啉单胞菌LPS（Pg LPS）以及Fn LPS和Pg LPS（Fn + Pg LPS）组。通过流式细胞术测定血液和气袋渗出液中的CD11bLy6G中性粒细胞和CD11bLy6GRANKL中性粒细胞。在血液中，与C组相比，Fn LPS组的CD11bLy6G中性粒细胞和CD11bLy6GRANKL中性粒细胞增多，而Pg LPS组无显著差异。Fn LPS组的这些增加与Ec LPS组无差异。在渗出液中，与C组相比，Fn LPS组和Pg LPS组的CD11bLy6G中性粒细胞和CD11bLy6GRANKL中性粒细胞增多。Fn LPS组的增加水平与Ec LPS组无差异，但Pg LPS组低于Ec LPS组。在血液和渗出液中，Fn + Pg LPS组与Ec LPS组相比，这些中性粒细胞水平无差异。具核梭杆菌和牙龈卟啉单胞菌的LPS增加了表达RANKL的中性粒细胞，尽管增加程度不同。这些表明牙周病原体LPS可作为刺激物增加表达RANKL的中性粒细胞。
