Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Georgia Institute of Technology, Atlanta, Georgia, USA.
J Clin Microbiol. 2021 Mar 19;59(4). doi: 10.1128/JCM.02355-20.
We evaluated the CLSI M44ed3E disk diffusion method compared with the CLSI M27ed4 broth microdilution method for caspofungin and fluconazole and the Etest method for amphotericin B to categorize susceptibility of 347 clinical isolates of Utilizing the zone diameter cutoffs established here, we observed overall categorical agreement between the two methods. For caspofungin, concordant results were observed for 98% of isolates, with <1% very major and 1% major errors. For fluconazole, concordant results were observed for 91% of isolates, with 1% very major and 8% major errors. For amphotericin B, concordant results were observed for 74% of isolates, with <1% very major errors and 25% major errors. The disk diffusion approach provides an accurate method for determining the susceptibility of for caspofungin and fluconazole and for identification of at least 75% of amphotericin B-susceptible isolates.
我们评估了 CLSI M44ed3E 纸片扩散法与 CLSI M27ed4 肉汤微量稀释法检测卡泊芬净和氟康唑的药敏性，以及 Etest 法检测两性霉素 B 的药敏性，以对 347 株临床分离株进行分类。利用这里建立的抑菌圈直径截断值，我们观察到两种方法之间的总体分类一致性。对于卡泊芬净，98%的分离株的结果一致，仅有<1%的非常重大错误和 1%的重大错误。对于氟康唑，91%的分离株的结果一致，仅有 1%的非常重大错误和 8%的重大错误。对于两性霉素 B，74%的分离株的结果一致，仅有<1%的非常重大错误和 25%的重大错误。纸片扩散法是一种准确的方法，可用于确定卡泊芬净和氟康唑的药敏性，并鉴定至少 75%的两性霉素 B 敏感的分离株。
