Wang Chen, Zhou Ying, Li Wei, Liu Xiaohu, Xi Ling, Li Pengcheng, Wei Juncheng, Lu Jinling
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Biomed Opt Express. 2020 Nov 11;11(12):7120-7131. doi: 10.1364/BOE.410394. eCollection 2020 Dec 1.
To examine the cervical lesions by using the tumor-targeted near-infrared (NIR) fluorescent dyes TMTP1-PEG4-ICG, a dual modal colposcope with visible reflectance imaging and fluorescence imaging was developed. NIR fluorescence imaging and visible light reflectance imaging were integrated together on a colposcope by designing the specific optics and adopting a dual sensor charge coupled device (CCD) camera. Patients with squamous cell carcinoma, adenocarcinoma, cervical intraepithelial neoplasia (CIN) and cervicitis were examined using this dual modal colposcope to validate its potential of cervical cancer detection. Fluorescent dye TMTP1-PEG4-ICG was applied to the cervix 30 minutes before inspection. The fluorescence images were collected after wiping the unbound fluorescent dye using normal saline. Signal to back ratios (SBR) of the fluorescence images were analyzed and compared with the histological analysis. The results suggest that the fluorescent colposcope combined with tumor-specific near-infrared fluorescent dyes TMTP1-PEG4-ICG could help to evaluate cervical lesions in real time.
为了使用肿瘤靶向近红外（NIR）荧光染料TMTP1-PEG4-ICG检查宫颈病变，开发了一种具有可见光反射成像和荧光成像功能的双模态阴道镜。通过设计特定光学系统并采用双传感器电荷耦合器件（CCD）相机，将NIR荧光成像和可见光反射成像集成到一台阴道镜上。使用这种双模态阴道镜对鳞状细胞癌、腺癌、宫颈上皮内瘤变（CIN）和宫颈炎患者进行检查，以验证其在宫颈癌检测方面的潜力。在检查前30分钟将荧光染料TMTP1-PEG4-ICG应用于宫颈。用生理盐水擦去未结合的荧光染料后收集荧光图像。分析荧光图像的信号与背景比（SBR），并与组织学分析结果进行比较。结果表明，结合肿瘤特异性近红外荧光染料TMTP1-PEG4-ICG的荧光阴道镜有助于实时评估宫颈病变。
