CAS Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory of Functional Molecular Imaging Technology, Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
University of Science and Technology of China, Hefei 230036, China.
J Am Chem Soc. 2020 Sep 2;142(35):14789-14804. doi: 10.1021/jacs.0c07022. Epub 2020 Aug 21.
Fluorescence imaging has become a fundamental tool for biomedical applications; nevertheless, its intravital imaging capacity in the conventional wavelength range (400-950 nm) has been restricted by its extremely limited tissue penetration. To tackle this challenge, a novel imaging approach using the fluorescence in the second near-infrared window (NIR-II, 1000-1700 nm) has been developed in the past decade to achieve deep penetration and high-fidelity imaging, and thus significant biomedical applications have begun to emerge. In this Perspective, we first examine recent discoveries and challenges in the development of novel NIR-II fluorophores and compatible imaging apparatuses. Subsequently, the recent advances in bioimaging, biosensing, and therapy using such a cutting-edge imaging technique are highlighted. Finally, based on the achievement in the representative studies, we elucidate the main concerns regarding this imaging technique and give some advice and prospects for the development of NIR-II imaging for future biomedical applications.
荧光成像是生物医学应用的基本工具；然而，其在传统波长范围（400-950nm）的活体成像能力受到其极有限的组织穿透性的限制。为了解决这一挑战，在过去十年中开发了一种使用近红外二区（NIR-II，1000-1700nm）荧光的新型成像方法，以实现深穿透和高保真成像，因此开始出现重大的生物医学应用。在本观点中，我们首先研究了新型 NIR-II 荧光团和兼容成像设备的发展中的最新发现和挑战。随后，强调了使用这种尖端成像技术在生物成像、生物传感和治疗方面的最新进展。最后，根据代表性研究的成果，我们阐明了对该成像技术的主要关注，并为未来生物医学应用中 NIR-II 成像的发展提出了一些建议和展望。
