Aziz Sahar Abdel Aleem Abdel, Mohamed Manar Bahaa El Din
Department of Animal Hygiene, Zoonosis and Epidemiology, Veterinary Medicine Faculty, Beni-Suef University, Egypt.
J Adv Vet Anim Res. 2020 Dec 3;7(4):710-717. doi: 10.5455/javar.2020.g472. eCollection 2020 Dec.
This study investigated the prevalence of in retail poultry shops, characterized the antibiotic resistance profile, and detected the genotypic pattern of virulence genes.
Broiler meat ( = 90), intestinal content ( = 40), and environmental samples ( = 95) were collected for this study. Besides, hand swabs ( = 20) were obtained from the poultry shop workers and stool samples ( = 40) were collected from the outpatient clinics of Beni-Suef University Hospital, Egypt. The samples were subjected to isolation and identification of by conventional bacteriological examinations and biochemical tests, followed by confirmatory identification by the polymerase chain reaction.
Among the collected samples ( 285), could be detected in 14.4% ( = 41/285) of the samples, where 30.0% ( = 12/40) of the intestinal content was positive. Similarly, 10.0% ( = 9/90), 15.0% ( = 3/20), and 12.5% ( = 5/40) of the samples of meat, hand swabs, and stools were found positive for , respectively. A total of 12 (12.6%) out of 95 environmental samples were positive for . Based on the antimicrobial sensitivity profile, most of the recovered isolates were multidrug-resistant against most commonly used antibiotics.
The findings conclude that poultry shops play a vital role in transmitting to the consumers. Asymptomatic poultry shop workers should draw attention to their potentials for spreading the infection to the consumers through the contaminated carcasses. Low hygienic standards are present in commercial poultry shops that increase the risk of contamination in the sold products.
本研究调查了零售家禽店中[具体细菌名称未给出]的流行情况，分析了其抗生素耐药谱，并检测了毒力基因的基因型模式。
本研究收集了90份鸡肉样本、40份肠道内容物样本和95份环境样本。此外，还从家禽店工作人员处采集了20份手部拭子样本，并从埃及贝尼苏韦夫大学医院的门诊收集了40份粪便样本。通过常规细菌学检查和生化试验对样本进行[具体细菌名称未给出]的分离和鉴定，随后通过聚合酶链反应进行确证鉴定。
在所收集的285份样本中，14.4%（41/285）的样本检测到[具体细菌名称未给出]，其中30.0%（12/40）的肠道内容物样本呈阳性。同样，鸡肉样本、手部拭子样本和粪便样本中分别有10.0%（9/90）、15.0%（3/20）和12.5%（5/40）检测为[具体细菌名称未给出]阳性。95份环境样本中共有12份（12.6%）呈[具体细菌名称未给出]阳性。根据抗菌药物敏感性分析，大多数分离菌株对最常用的抗生素具有多重耐药性。
研究结果表明，家禽店在将[具体细菌名称未给出]传播给消费者方面起着至关重要的作用。无症状的家禽店工作人员应注意其通过受污染的 carcasses 将感染传播给消费者的可能性。商业家禽店卫生标准较低，增加了所售产品受污染的风险。
