Laboratory of Edible Fungi, Institute of Technology of Agricultural Products (ITAP), Hellenic Agricultural Organization - Demeter, 1 Sofokli Venizelou Street, 14123 - Lykovryssi, Attiki, Greece.
Laboratory of Food Chemistry and Analysis, Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855, Athens, Greece.
Environ Sci Pollut Res Int. 2021 Apr;28(16):20570-20585. doi: 10.1007/s11356-020-11835-4. Epub 2021 Jan 6.
A new wild-type Ganoderma resinaceum isolate was cultivated on glucose-enriched liquid cultures with olive mill wastewaters (OMWs) in initial phenolic compounds concentrations 0.0 (control), 0.5, 0.8, and 1.5 g/l. The effect of the fungus on the reduction of phenolics and color was assessed, whereas biomass production, glucose consumption, intra-cellular (IPS) and extra-cellular (EPS) polysaccharides biosynthesis, antioxidant activity of the biomass, and laccase synthesis were monitored. Results showed that significant phenolic reduction (94.5%) and decolorization (76.5%) occurred, 14.6 g/l of biomass was produced, glucose was almost totally consumed, EPS were produced in sufficient amounts (0.79 g/l), whereas the presence of OMWs enhanced the synthesis of IPS (maximum absolute values 4.0-5.2 g/l corresponding to 35-42% w/w). Kinetic analysis demonstrated that EPS and IPS values fluctuated with time, regardless of the available amount of glucose in the media, showing a maximum in the 17th day of culture. Laccase was highly synthesized in the middle of the fermentation, reaching the maximum value of 14 U/ml. Little growth was however observed at 1.5 g/l phenolics. Strong correlation between total phenolic content and free radical scavenging activity has been noticed in the methanolic extracts of the mycelium. Results strongly suggest the potentiality of G. resinaceum utilization in the OMW waste treatment.
一种新的野生灵芝分离株在富含葡萄糖的液体培养基中用橄榄厂废水（OMWs）进行培养，初始酚类化合物浓度分别为 0.0（对照）、0.5、0.8 和 1.5 g/L。评估了真菌对酚类物质和颜色减少的影响，同时监测了生物量生产、葡萄糖消耗、细胞内（IPS）和细胞外（EPS）多糖生物合成、生物质抗氧化活性和漆酶合成。结果表明，酚类物质的去除率达到 94.5%，脱色率达到 76.5%，生物量达到 14.6 g/L，葡萄糖几乎完全被消耗，EPS 产量充足（0.79 g/L），而 OMWs 的存在增强了 IPS 的合成（最大绝对值为 4.0-5.2 g/L，对应 35-42% w/w）。动力学分析表明，EPS 和 IPS 值随时间波动，与培养基中葡萄糖的可用量无关，在培养的第 17 天达到最大值。漆酶在发酵中期高度合成，达到 14 U/ml 的最大值。然而，在 1.5 g/L 酚类物质下，生长很少。在菌丝体的甲醇提取物中观察到总酚含量与自由基清除活性之间存在很强的相关性。结果强烈表明灵芝在处理 OMW 废物方面的潜力。
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