Department of Entomology, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China.
MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
EMBO Rep. 2021 Feb 3;22(2):e47910. doi: 10.15252/embr.201947910. Epub 2021 Jan 7.
Sleep homeostasis is crucial for sleep regulation. The role of epigenetic regulation in sleep homeostasis is unestablished. Previous studies showed that octopamine is important for sleep homeostasis. However, the regulatory mechanism of octopamine reception in sleep is unknown. In this study, we identify an epigenetic regulatory cascade (Stuxnet-Polycomb-Octβ2R) that modulates the octopamine receptor in Drosophila. We demonstrate that stuxnet positively regulates Octβ2R through repression of Polycomb in the ellipsoid body of the adult fly brain and that Octβ2R is one of the major receptors mediating octopamine function in sleep homeostasis. In response to octopamine, Octβ2R transcription is inhibited as a result of stuxnet downregulation. This feedback through the Stuxnet-Polycomb-Octβ2R cascade is crucial for sleep homeostasis regulation. This study demonstrates a Stuxnet-Polycomb-Octβ2R-mediated epigenetic regulatory mechanism for octopamine reception, thus providing an example of epigenetic regulation of sleep homeostasis.
睡眠内稳态对于睡眠调节至关重要。表观遗传调控在睡眠内稳态中的作用尚未确定。先前的研究表明，章鱼胺对于睡眠内稳态很重要。然而，章鱼胺受体在睡眠中的调控机制尚不清楚。在这项研究中，我们鉴定了一个表观遗传调控级联（Stuxnet-Polycomb-Octβ2R），它调节果蝇中的章鱼胺受体。我们证明，Stuxnet 通过抑制成年果蝇脑的椭圆体中的 Polycomb 来正向调节 Octβ2R，并且 Octβ2R 是介导章鱼胺在睡眠内稳态中作用的主要受体之一。对章鱼胺的反应，由于 Stuxnet 的下调，Octβ2R 的转录被抑制。通过 Stuxnet-Polycomb-Octβ2R 级联的这种反馈对于睡眠内稳态的调节至关重要。这项研究证明了章鱼胺受体的 Stuxnet-Polycomb-Octβ2R 介导的表观遗传调控机制，从而为睡眠内稳态的表观遗传调控提供了一个范例。
