静水压力调节的细胞钙反应

Hydrostatic Pressure-Regulated Cellular Calcium Responses.

作者信息

Fukuchi Minami, Oyama Kotaro, Mizuno Hiroaki, Miyagawa Akihisa, Koumoto Kazuya, Fukuhara Gaku

机构信息

Department of Chemistry, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8551, Japan.

Takasaki Advanced Radiation Research Institute, National Institutes for Quantum and Radiological Science and Technology (QST), 1233 Watanukimachi, Takasaki, Gunma 370-1292, Japan.

出版信息

Langmuir. 2021 Jan 19;37(2):820-826. doi: 10.1021/acs.langmuir.0c03141. Epub 2021 Jan 7.

DOI:10.1021/acs.langmuir.0c03141

PMID:33410684

Abstract

Hydrostatic pressure control has attracted much attention and presents a still challenging objective from mechanobiological viewpoints. Herein, we reveal the calcium entry processes in HeLa cells by means of hydrostatic pressure spectroscopy. The steady-state fluorescence spectral data comprehensively elucidated the factors controlling the outcomes of the hydrostatic pressure-stimulated calcium entry behavior. The present work leads to a new perspective on ion regulations in living cells and an attractive alternative to conventional mechanostimuli.

摘要

静水压力控制已引起广泛关注，从力学生物学角度来看，这仍是一个具有挑战性的目标。在此，我们借助静水压力光谱法揭示了HeLa细胞中的钙内流过程。稳态荧光光谱数据全面阐明了控制静水压力刺激钙内流行为结果的因素。目前的工作为活细胞中的离子调节带来了新的视角，并且是传统机械刺激的一个有吸引力的替代方法。