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富含丙氨酸的肽的作用机制和肽-膜相互作用的深入了解：多技术实验和理论分析。

Insight into the Mechanism of Action and Peptide-Membrane Interactions of Aib-Rich Peptides: Multitechnique Experimental and Theoretical Analysis.

机构信息

Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.

ISIS Neutron and Muon Source, Rutherford Appleton Laboratory Harwell Didcot, Oxford, OX11 0QX, UK.

出版信息

Chembiochem. 2021 May 4;22(9):1656-1667. doi: 10.1002/cbic.202000834. Epub 2021 Feb 24.

DOI:10.1002/cbic.202000834
PMID:33411956
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8248331/
Abstract

The increase in resistant bacterial strains necessitates the identification of new antimicrobial molecules. Antimicrobial peptides (AMPs) are an attractive option because of evidence that bacteria cannot easily develop resistance to AMPs. The peptaibols, a class of naturally occurring AMPs, have shown particular promise as antimicrobial drugs, but their development has been hindered by their mechanism of action not being clearly understood. To explore how peptaibols might interact with membranes, circular dichroism, vibrational circular dichroism, linear dichroism, Raman spectroscopy, Raman optical activity, neutron reflectivity and molecular dynamics simulations have been used to study a small library of peptaibol mimics, the Aib-rich peptides. All the peptides studied quickly partitioned and oriented in membranes, and we found evidence of chiral interactions between the phospholipids and membrane-embedded peptides. The protocols presented in this paper open new ground by showing how chiro-optical spectroscopies can throw light on the mechanism of action of AMPs.

摘要

耐药菌株的增加需要鉴定新的抗菌分子。由于有证据表明细菌不容易对 AMP 产生耐药性，因此抗菌肽 (AMPs) 是一个有吸引力的选择。肽类抗生素是一类天然存在的 AMPs，作为抗菌药物具有特别的前景，但由于其作用机制尚不清楚，其发展受到了阻碍。为了探索肽类抗生素可能与膜相互作用的方式，圆二色性、振动圆二色性、线二色性、拉曼光谱、拉曼光学活性、中子反射率和分子动力学模拟已被用于研究一小部分肽类抗生素模拟物，即富含丙氨酸的肽。所有研究的肽都能快速分配和在膜中定向，并且我们发现了磷脂和膜嵌入肽之间手性相互作用的证据。本文提出的方案通过展示手性光学光谱如何阐明 AMPs 的作用机制，开辟了新的研究途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/e9bfc7c077c5/CBIC-22-1656-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/da0cc685da9b/CBIC-22-1656-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/05ab13e60151/CBIC-22-1656-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/816ef642c839/CBIC-22-1656-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/6a069c731e9c/CBIC-22-1656-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/7d5aac9b0b3e/CBIC-22-1656-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/0e10ad414a57/CBIC-22-1656-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/d7bf681b2657/CBIC-22-1656-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/c4ca82bb9b40/CBIC-22-1656-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/e9bfc7c077c5/CBIC-22-1656-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/da0cc685da9b/CBIC-22-1656-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/05ab13e60151/CBIC-22-1656-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/816ef642c839/CBIC-22-1656-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/6a069c731e9c/CBIC-22-1656-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/7d5aac9b0b3e/CBIC-22-1656-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/0e10ad414a57/CBIC-22-1656-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/d7bf681b2657/CBIC-22-1656-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/c4ca82bb9b40/CBIC-22-1656-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe90/8248331/e9bfc7c077c5/CBIC-22-1656-g010.jpg

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Int J Mol Sci. 2023 Mar 14;24(6):5537. doi: 10.3390/ijms24065537.
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J Am Chem Soc. 2022 Nov 30;144(47):21648-21657. doi: 10.1021/jacs.2c09103. Epub 2022 Nov 15.
J Am Chem Soc. 2019 Dec 26;141(51):20460-20469. doi: 10.1021/jacs.9b11194. Epub 2019 Dec 10.
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