Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
Department of Phytochemistry, National Organization for Drug Control and Research, Giza, Egypt.
J Ethnopharmacol. 2021 Apr 24;270:113777. doi: 10.1016/j.jep.2021.113777. Epub 2021 Jan 4.
ETHNO-PHARMACOLOGICAL RELEVANCE: Artemisia judaica L is an aromatic medicinal plant growing widely in Saint Katherine, Sinai, Egypt, and used in traditional medicine as a herbal remedy for antibacterial, anthelmintic, antidiabetic, analgesic and anti-inflammatory activities. Additionally, other Arabic regions commonly used it in their folk medicines for the treatment of fungal infections, atherosclerosis, cancer, diabetes, arthritis, and inflammatory-related diseases.
Based on the traditional medicinal uses of A. judaica, the present study was designed to validate some of the traditional uses as the analgesic, anti-inflammatory, antipyretic, hepatoprotective, antidiabetic, and antioxidant activities of 80% aqueous methanol extract (AME) of A. judaica aerial parts as well as isolation and identification of its flavonoid content.
AME of A. judaica aerial parts was fractionated using column chromatography and the structures of the isolated compounds were established using different spectroscopic data. Analgesic activity was evaluated using acetic acid-induced writhing in mice; antipyretic activity was assessed using yeast suspension-induced hyperthermia in rats; anti-inflammatory activity was evaluated using carrageenan-induced paw edema; the hepatoprotective effect was studied by measuring liver enzymes in carbon tetrachloride(CCl)-induced hepatotoxicity rats while antidiabetic activity was estimated in alloxan hyperglycemia.
Eight flavone compounds namely luteolin 4' methyl ether 7-O-β-D-C-glucopyranoside (1), 8-methoxyapigenin 7-O-β-D-C-galactopyranoside (2), isovitexin (3), 8-methoxyluteolin 7-O-β-D-C-glucopyranoside (4), diosmetin (5), cirsimaritin (6), luteolin (7), and apigenin (8) were identified from AME of A. judaica. The AME was found to be non-toxic to mice up to 5 g/kg b.w. Moreover, it exhibits significant analgesic antipyretic, anti-inflammatory, antidiabetic, hepatoprotective, and antioxidant activities in a dose-dependent manner.
The AME was nontoxic; it exhibits significant analgesic, antipyretic, anti-inflammatory, antidiabetic, hepatoprotective, and antioxidant activities. Moreover, the isolated flavone was identified from AME for the first time.
Artemisia judaica L 是一种芳香药用植物，广泛生长于埃及圣凯瑟琳山 Sinai，在传统医学中被用作草药治疗剂，具有抗菌、驱虫、抗糖尿病、镇痛和抗炎作用。此外，其他阿拉伯地区也常用其民间药物治疗真菌感染、动脉粥样硬化、癌症、糖尿病、关节炎和炎症相关疾病。
基于 Artemisia judaica 的传统药用用途，本研究旨在验证其一些传统用途，即 80%甲醇提取物（AME）的镇痛、抗炎、解热、保肝、抗糖尿病和抗氧化活性，以及分离和鉴定其类黄酮含量。
用柱层析法对 Artemisia judaica 地上部分的 AME 进行分离，根据不同的光谱数据确定分离化合物的结构。通过醋酸诱导的小鼠扭体试验评价镇痛活性；通过酵母混悬液诱导的大鼠发热试验评价解热活性；通过角叉菜胶诱导的足肿胀试验评价抗炎活性；通过四氯化碳（CCl）诱导的肝毒性大鼠测量肝酶来研究保肝作用，通过测定链脲佐菌素（alloxan）高血糖大鼠的血糖来评价抗糖尿病作用。
从 AME 中鉴定出 8 种类黄酮化合物，分别为木樨草素 4'-甲基醚 7-O-β-D-C-吡喃葡萄糖苷（1）、8-甲氧基芹菜素 7-O-β-D-C-半乳糖吡喃糖苷（2）、异荭草苷（3）、8-甲氧基木樨草素 7-O-β-D-吡喃葡萄糖苷（4）、地奥司明（5）、白当归素（6）、木樨草素（7）和芹菜素（8）。AME 对小鼠的最大耐受量（5g/kg）无毒性。此外，AME 呈剂量依赖性表现出显著的镇痛、解热、抗炎、抗糖尿病、保肝和抗氧化活性。
AME 无毒；具有显著的镇痛、解热、抗炎、抗糖尿病、保肝和抗氧化活性。此外，从 AME 中首次分离出类黄酮。
