Alanazi Naimah Asid H, Alhejely Amani, Areshi Sultan Mohammed, Alghibiwi Hanan K, Alhabardi Samiah A, Akeel Mohammed A, Alshammari Amal Naif, Alrajeh Sarah Mohammed, Al-Hamoud Gadah A, Salama Salama A
Department of Biology, College of Sciences, University of Ha'il, Ha'il 53962, Saudi Arabia.
Biology Department, Darb University College, Jazan University, Jazan 45142, Saudi Arabia.
Int J Mol Sci. 2025 Jul 30;26(15):7355. doi: 10.3390/ijms26157355.
Today's primary challenges include identifying efficient, affordable, and environmentally sustainable substances to serve as raw materials in industrial, agricultural, and medicinal applications. This study aimed to evaluate the chemical composition and biological properties (namely antioxidant and allelopathic activities) of the methanolic extract derived from the above-ground portions of collected in Jazan, Saudi Arabia. GC-MS was used to evaluate the chemical composition of the methanolic extract derived from . GC-MS analysis revealed a total of 22 volatile compounds in the extract. The most prominent compounds identified were 2-ethylhexanoic acid, 5-hydroxy-6-(1-hydroxyethyl)-2,7-dimethoxynaphtho-quinone, and piperitone. The extract demonstrated strong antioxidant activity in both the DPPH and ABTS radical scavenging assays, comparable to the standard antioxidant ascorbic acid. The IC value for the extract was 31.82 mg/mL in the DPPH assay and 39.93 mg/mL in the ABTS testing. Additionally, the extract exhibited dose-dependent inhibition of seed germination, root growth, and shoot growth of the weed in allelopathic bioassays. The most significant suppression was observed in shoot growth with an IC value of 45.90 mg/mL, which was lower than the IC values for root development and seed germination of , recorded at 56.16 mg/mL and 88.80 mg/mL, respectively. Furthermore, the findings indicated that methanolic extracts had significant lethal toxic effects on the life cycle of . Future research will focus on extracting uncontaminated substances and evaluating the biological effects of each specific constituent.
当今的主要挑战包括确定高效、经济且环境可持续的物质，以用作工业、农业和医药应用中的原材料。本研究旨在评估从沙特阿拉伯吉赞采集的[植物名称未给出]地上部分的甲醇提取物的化学成分和生物学特性（即抗氧化和化感活性）。采用气相色谱 - 质谱联用（GC - MS）法评估[植物名称未给出]甲醇提取物的化学成分。GC - MS分析显示提取物中共有22种挥发性化合物。鉴定出的最主要化合物为2 - 乙基己酸、5 - 羟基 - 6 -（1 - 羟乙基）- 2,7 - 二甲氧基萘醌和胡椒酮。该提取物在DPPH和ABTS自由基清除试验中均表现出较强的抗氧化活性，与标准抗氧化剂抗坏血酸相当。在DPPH试验中提取物的IC值为31.82 mg/mL，在ABTS试验中为39.93 mg/mL。此外，在化感生物测定中，该提取物对杂草[杂草名称未给出]的种子萌发、根生长和芽生长表现出剂量依赖性抑制作用。在芽生长中观察到最显著的抑制作用，IC值为45.90 mg/mL，低于[杂草名称未给出]根发育和种子萌发的IC值，分别为56.16 mg/mL和88.80 mg/mL。此外，研究结果表明甲醇提取物对[生物名称未给出]的生命周期具有显著的致死毒性作用。未来的研究将集中于提取未受污染的物质并评估每种特定成分的生物学效应。
