Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas-Instituto Politécnico Nacional, Cd. México, Mexico.
Functional Genomics of Prokaryotes, Center for Genomic Sciences, National University of Mexico, Cuernavaca, Morelos, Mexico.
Res Microbiol. 2021 Mar;172(2):103796. doi: 10.1016/j.resmic.2020.103796. Epub 2021 Jan 5.
Previous studies have shown that biofilm-forming bacteria are deficient in tricarboxylic acid (TCA) cycle metabolites, suggesting a relationship between these cellular processes. In this work, we compared the proteomes of planktonic vs biofilm cells from a clinical strain of Staphylococcus epidermidis using LC-MS/MS. A total of 168 proteins were identified from both growth conditions. The biofilm cells showed enrichment of proteins participating in glycolysis for the formation of pyruvate; however, the absence of TCA cycle proteins and the presence of lactate dehydrogenase, formate acetyltransferase, and acetoin reductase suggested that pyruvate was catabolized to their respective products: lactate, formate and acetoin. On the other hand, planktonic cells showed proteins participating in glycolysis and the TCA cycle, the pentose phosphate pathway, gluconeogenesis, ATP generation and the oxidative stress response. Functional networks with higher interconnection were predicted for planktonic proteins. We propose that in S. epidermidis, the relative absence of TCA cycle proteins is associated with the formation of biofilms and that lactate, formate and acetoin are the end products of partial glucose metabolism.
先前的研究表明，生物膜形成细菌中三羧酸 (TCA) 循环代谢物缺乏，提示这些细胞过程之间存在关系。在这项工作中，我们使用 LC-MS/MS 比较了表皮葡萄球菌临床株浮游细胞和生物膜细胞的蛋白质组。从两种生长条件中共鉴定出 168 种蛋白质。生物膜细胞中参与糖酵解以形成丙酮酸的蛋白质富集；然而，TCA 循环蛋白的缺失和乳酸脱氢酶、甲酸乙酰转移酶和乙酰丁醇还原酶的存在表明丙酮酸被代谢为各自的产物：乳酸、甲酸和乙酰丁醇。另一方面，浮游细胞显示出参与糖酵解和 TCA 循环、戊糖磷酸途径、糖异生、ATP 生成和氧化应激反应的蛋白质。预测浮游细胞的功能网络具有更高的连接度。我们提出，在表皮葡萄球菌中，TCA 循环蛋白的相对缺乏与生物膜的形成有关，而乳酸、甲酸和乙酰丁醇是部分葡萄糖代谢的终产物。
