Department of Aquaculture, Faculty of Fisheries, Ataturk University, Erzurum, Turkey.
Department of Nutrition and Dietetics, Faculty of Health Sciences, Ataturk University, Erzurum, Turkey.
Toxicol Mech Methods. 2021 Mar;31(3):224-234. doi: 10.1080/15376516.2021.1871794. Epub 2021 Feb 11.
Pesticide toxicities are common in aquatic ecosystems and affects aquatic livings negative. Therefore, it is important to strengthen the antioxidant system in aquatic organisms and to protect the organisms against these toxic chemicals. In this study, the simulative toxicity was established to the fish then the healing process was followed. For this purpose, rainbow trout exposed to cypermethrin and left to the recovery process with either N-acetyl cysteine (an antioxidant, 0.5 mM-1.0 mM concentrations) or no intervention (self-healing) for 96 h. In this context, paraoxonase (PON), arylesterase (AR), myeloperoxidase (MPO), antioxidant enzymes (SOD, CAT, GPx), acetylcholinesterase (AChE) activities as well as MDA, caspase-3 and 8-OHdG levels were measured in fish gills, liver and kidney tissues. In addition, trace element tests were performed in the tissues sampled for each group. At the result of pesticide exposure, SOD, CAT, GPx, PON, AR and AChE activities were increased but MDA, MPO, caspase-3 and 8-OHdG levels were decreased in N-acetyl cysteine (NAC) treated groups in all tissues compared to self-healing group ( < 0.05). When the element analysis of the samples was examined, tissue-based differences were observed significantly in all application groups ( < 0.05). Considering the results of the study, it was found that NAC administration at high concentration (1.0 Mm NAC) was more effective on pesticide toxicity. It was concluded that the most sensitive tissue was the kidney.
在水生生态系统中，农药毒性很常见，对水生生物有负面影响。因此，加强水生生物的抗氧化系统，保护生物免受这些有毒化学物质的侵害非常重要。在这项研究中，对鱼类进行了模拟毒性实验，然后观察了其恢复过程。为此，将虹鳟鱼暴露于氯菊酯中，然后让其在恢复过程中自行恢复（自我修复）或用 N-乙酰半胱氨酸（抗氧化剂，0.5 mM-1.0 mM 浓度）进行干预 96 小时。在这种情况下，在鱼鳃、肝脏和肾脏组织中测量了对氧磷酶（PON）、芳基酯酶（AR）、髓过氧化物酶（MPO）、抗氧化酶（SOD、CAT、GPx）、乙酰胆碱酯酶（AChE）的活性以及 MDA、caspase-3 和 8-OHdG 的水平。此外，还对每组采样的组织进行了微量元素测试。在农药暴露的结果中，与自我修复组相比，N-乙酰半胱氨酸（NAC）处理组所有组织中的 SOD、CAT、GPx、PON、AR 和 AChE 活性增加，但 MDA、MPO、caspase-3 和 8-OHdG 水平降低（<0.05）。当检查样本的元素分析时，在所有应用组中都观察到组织之间存在显著差异（<0.05）。考虑到研究结果，发现高浓度（1.0 mM NAC）的 NAC 给药对农药毒性更有效。研究结论是，最敏感的组织是肾脏。
