Department of Aquaculture, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey.
Department of Chemistry, Faculty of Science, University of Yüzüncü Yıl, TR-65080, Van, Turkey.
Chemosphere. 2019 Apr;221:30-36. doi: 10.1016/j.chemosphere.2018.12.196. Epub 2019 Jan 4.
The aim of this study was to investigate the therapeutic effect of N-acetylcystein (NAC) against oxidative stress induced by Cypermethrin pesticide in rainbow trout (Oncorhynchus mykiss). The experiment was designed as 5 groups (A, B, C, D, and E). Group A was organized as control group and had no treatment. The other groups were treated with Cypermethrin for 14 days. At the end of this period, Groups B (1.0 mM NAC) and D (0.5 mM NAC) was performed with NAC for 96 h. Group C was not administered NAC, the recovery process was evaluated with this group. Group E was exposed to cypermethrin during 14 days and sampled. Acetylcholinesterase (AChE), malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), paraoxonase (PON), arylesterase (AR), myeloperoxidase (MPO) activities, oxidative DNA damage (8-hydroxy-2'-deoxyguanosine (8-OHdG)), caspase-3 levels, and trace elements contents analyses were performed in all fish brains. According to the results, MDA, MPO, 8-OHdG and caspase-3 levels were significantly decreased compared to the other groups (pesticide and recovery) (p < 0.05), AChE, SOD, CAT, GPx, PON, and AR activities increased (p < 0.05). In brain tissue, no statistically significant difference was observed in trace element analysis of all application groups. According to the obtained data, the positive effect of N-acetylcysteine on protein synthesis, detoxification, and diverse metabolic functions against cypermethrin toxicity has been more effective in 1.0 mM NAC. NAC has important therapeutic effect on pesticide-induced neurotoxicity for fish in terms of all data. It was concluded that NAC has an antioxidant effect against pesticide-induced oxidative stress and the selected biochemical markers are useful for such studies.
本研究旨在探讨 N-乙酰半胱氨酸（NAC）对拟除虫菊酯农药诱导的虹鳟鱼（Oncorhynchus mykiss）氧化应激的治疗作用。实验设计为 5 组（A、B、C、D 和 E）。A 组为对照组，无处理。其余各组用拟除虫菊酯处理 14 天。在这一时期结束时，B 组（1.0 mM NAC）和 D 组（0.5 mM NAC）用 NAC 处理 96 h。C 组未给予 NAC，用该组评估恢复过程。E 组在 14 天内暴露于拟除虫菊酯并取样。对所有鱼脑进行乙酰胆碱酯酶（AChE）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、对氧磷酶（PON）、芳基酯酶（AR）、髓过氧化物酶（MPO）活性、氧化 DNA 损伤（8-羟基-2'-脱氧鸟苷（8-OHdG））、caspase-3 水平和微量元素含量分析。结果表明，与其他组（农药和恢复组）相比，MDA、MPO、8-OHdG 和 caspase-3 水平显著降低（p<0.05），AChE、SOD、CAT、GPx、PON 和 AR 活性增加（p<0.05）。在脑组织中，所有应用组的微量元素分析均无统计学差异。根据获得的数据，1.0 mM NAC 对 N-乙酰半胱氨酸对蛋白质合成、解毒和多种代谢功能的积极影响对拟除虫菊酯毒性更有效。NAC 对鱼类因农药引起的神经毒性具有重要的治疗作用，所有数据均表明这一点。结论是，NAC 对农药诱导的氧化应激具有抗氧化作用，所选生化标志物可用于此类研究。
