The Intractable Diseases Diagnosis and Treatment Center for Liver, Gallbladder, Pancreas & Intestine, Department of Hepatopancreatobiliary Surgery, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, Hunan Province, People's Republic of China.
Technol Cancer Res Treat. 2021 Jan-Dec;20:1533033820983302. doi: 10.1177/1533033820983302.
Emerging evidence has shown that circular RNAs (circRNAs) serve as a promising biomarker in different malignancies. Specifically, circ_001569 has been found to be upregulated in some types of human gastrointestinal cancer. In this study, we aimed to investigate clinical significances, diagnostic and prognostic values of circ_001569 in pancreatic cancer (PC), and identify its effects on the malignant behaviors of PC cells. The expression of circ_001569 was determined in 26 tissues samples and 97 plasma samples from PC patients by qRT-PCR. Relationship between circ_001569 expression and clinicopathological parameters was analyzed by Chi-square test. Diagnostic and prognostic values of circ_001569 were evaluated by ROC curves, Kaplan-Meier curves, and Cox regression analysis. The effects of circ_001569 on the proliferation, migration, invasion, and apoptosis of PC cells were assessed by MTT, wound healing, Transwell invasion assays, and flow cytometric analysis, respectively. Results showed that the expression of circ_001569 was upregulated in tissues and plasma of PC patients. High circ_001569 level was positively correlated with lymphatic metastasis, clinical stage, and venous invasion. Circ_001569 level was an independent prognostic indicator for overall survival rates of PC patients, and patients with high circ_001569 level had a poor prognosis. The AUC of circ_001569 was 0.716 (95% CI: 0.642-0.790) with a sensitivity and specificity of 62.76% and 74.29%, respectively. , circ_001569 silencing decreased cell proliferation, migration, and invasion, but promoted cell apoptosis of PC cells. Our data demonstrate that high circ_001569 level associates with tumor malignant behaviors, and may serve as a potential biomarker in the diagnosis and prognosis of PC.
越来越多的证据表明，环状 RNA（circRNAs）作为一种有前途的生物标志物在不同的恶性肿瘤中发挥作用。具体来说，circ_001569 在一些类型的人类胃肠道癌中被发现上调。在这项研究中，我们旨在研究 circ_001569 在胰腺癌（PC）中的临床意义、诊断和预后价值，并确定其对 PC 细胞恶性行为的影响。通过 qRT-PCR 检测 26 个组织样本和 97 个 PC 患者的血浆样本中的 circ_001569 表达。通过卡方检验分析 circ_001569 表达与临床病理参数的关系。通过 ROC 曲线、Kaplan-Meier 曲线和 Cox 回归分析评估 circ_001569 的诊断和预后价值。通过 MTT、划痕愈合、Transwell 侵袭实验和流式细胞术分析分别评估 circ_001569 对 PC 细胞增殖、迁移、侵袭和凋亡的影响。结果表明，circ_001569 在 PC 患者的组织和血浆中表达上调。高 circ_001569 水平与淋巴转移、临床分期和静脉侵犯呈正相关。circ_001569 水平是 PC 患者总生存率的独立预后指标，高 circ_001569 水平的患者预后不良。circ_001569 的 AUC 为 0.716（95%CI：0.642-0.790），灵敏度和特异性分别为 62.76%和 74.29%。circ_001569 沉默降低了 PC 细胞的增殖、迁移和侵袭，但促进了细胞凋亡。我们的数据表明，高 circ_001569 水平与肿瘤恶性行为相关，可能作为 PC 诊断和预后的潜在生物标志物。
