环状 RNA circRHOT1 在胰腺癌中上调，并促进细胞增殖和侵袭。
Circular RNA circRHOT1 is upregulated and promotes cell proliferation and invasion in pancreatic cancer.
机构信息
Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, PR China.
Department of General Surgery, 141 Hospital, Xi'an, PR China.
出版信息
Epigenomics. 2019 Jan;11(1):53-63. doi: 10.2217/epi-2018-0051. Epub 2018 Nov 16.
AIM
We aimed to identify the roles of circRHOT1 in pancreatic cancer.
MATERIALS & METHODS: The circRHOT1 was acquired from our previous study followed by quantitative real-time PCR and fluorescence in situ hybridization validation in pancreatic cancer. We used siRNA and shRNA to explore the function of circRHOT1 in pancreatic cancer cells. Bioinformatic analyses were applied to study the potential mechanism of circRHOT1.
RESULTS
The circRHOT1 was upregulated in pancreatic cancer and predominantly located in the cytoplasm. Reducing the circRHOT1 expression may inhibit the pancreatic cancer cell proliferation, invasion and migration. The circRHOT1 may play a role in pancreatic cancer through binding miR-26b, miR-125a, miR-330 and miR-382 to regulate multiple tumor-associated pathways.
CONCLUSION
This study demonstrated that circRHOT1 may serve as an oncogenic circRNA that promotes tumor progression.
目的
本研究旨在探讨环状 RNA（circRHOT1）在胰腺癌中的作用。
材料与方法
circRHOT1 由本课题组前期研究获得，通过实时定量 PCR 和荧光原位杂交实验对其在胰腺癌中的表达进行验证。利用 siRNA 和 shRNA 干扰技术探讨 circRHOT1 对胰腺癌细胞功能的影响。应用生物信息学分析方法探讨 circRHOT1 的潜在作用机制。
结果
circRHOT1 在胰腺癌中呈高表达，且主要定位于细胞质。降低 circRHOT1 的表达可抑制胰腺癌细胞的增殖、侵袭和迁移。circRHOT1 可能通过结合 miR-26b、miR-125a、miR-330 和 miR-382 调控多个肿瘤相关通路发挥致癌作用。
结论
本研究表明 circRHOT1 可能作为一种致癌环状 RNA 促进肿瘤进展。
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