Chen Yuan, Geng Yiting, Huang Junchao, Xi Dan, Xu Guoping, Gu Wendong, Shao Yingjie
Department of Radiation Oncology, The Third Affiliated Hospital of Soochow University, 185 Juqian Street, Changzhou, 213003, China.
Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, 213003, China.
Cancer Cell Int. 2021 Jan 7;21(1):34. doi: 10.1186/s12935-020-01736-4.
CircRNAs play crucial roles in multiple tumours. However, the functions of most circRNAs in cervical cancer remain unclear.
This study collected GSE113696 data from the GEO database to search for differentially expressed circRNAs in cervical cancer. Quantitative reverse transcription PCR was used to detect the expression level of circNEIL3 in cervical cancer cells and tissues. Then, functional experiments in vitro and in vivo were performed to evaluate the effects of circNEIL3 in cervical cancer.
CircNEIL3 was highly expressed in cervical cancer. In vivo and in vitro experiments verified that circNEIL3 enhanced the proliferation capacity of cervical cancer cells. RNA immunoprecipitation, luciferase reporter assay, pull-down assay, and fluorescent in situ hybridization confirmed the interaction between circNEIL3 and miR-137 in cervical cancer. A luciferase reporter assay showed that circNEIL3 adsorbed miR-137 and upregulated KLF12 to regulate the proliferation of cervical cancer cells.
CircNEIL3 is an oncogene in cervical cancer and might serve as a ceRNA that competitively binds to miR-137, thereby indirectly upregulating the expression of KLF12 and promoting the proliferation of cervical cancer cells.
环状RNA（circRNAs）在多种肿瘤中发挥关键作用。然而，大多数circRNAs在宫颈癌中的功能仍不清楚。
本研究从基因表达综合数据库（GEO数据库）收集GSE113696数据，以寻找宫颈癌中差异表达的circRNAs。采用定量逆转录PCR检测circNEIL3在宫颈癌细胞和组织中的表达水平。然后，进行体内和体外功能实验，以评估circNEIL3在宫颈癌中的作用。
circNEIL3在宫颈癌中高表达。体内和体外实验证实，circNEIL3增强了宫颈癌细胞的增殖能力。RNA免疫沉淀、荧光素酶报告基因检测、下拉实验和荧光原位杂交证实了circNEIL3与宫颈癌中miR-137之间的相互作用。荧光素酶报告基因检测表明，circNEIL3吸附miR-137并上调KLF12，从而调节宫颈癌细胞的增殖。
circNEIL3是宫颈癌中的一种癌基因，可能作为一种竞争性内源RNA（ceRNA）与miR-137竞争性结合，从而间接上调KLF12的表达并促进宫颈癌细胞的增殖。
