信号转导及转录激活因子3（STAT3）通过激活WDHD1在DNA复制中发挥重要作用。

STAT3 plays an important role in DNA replication by turning on WDHD1.

作者信息

Zhou Yunying, Chen Jason J

机构信息

Medical Research & Laboratory Diagnostic Center, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.

Medical Research & Laboratory Diagnostic Center, Central Hospital Affiliated To Shandong First Medical University, Jinan, China.

出版信息

Cell Biosci. 2021 Jan 7;11(1):10. doi: 10.1186/s13578-020-00524-x.

BACKGROUND

Signal transducers and activators of transcription 3 (STAT3) is a transcription factor that plays a key role in many cellular processes such as cell growth and cancer. However, the functions and mechanisms by which STAT3 regulates cellular processes are not fully understood.

RESULTS

Here we describe a novel function of STAT3. We demonstrated that STAT3 plays an important role in DNA replication. Specifically, knockdown of STAT3 reduced DNA replication while activation and ectopic expression of STAT3 promoted DNA replication. We further identified the WD repeat and HMG-box DNA-binding protein 1 (WDHD1), which plays an important role in DNA replication initiation, as a novel STAT3 target gene that mediated the DNA replication function of STAT3. We showed that STAT3 bind the promoter/up regulatory region of WDHD1 gene.

CONCLUSIONS

These studies identified a novel function of STAT3 that is mediated by its newly identified target gene WDHD1 and have important implications.

背景

信号转导子和转录激活子3（STAT3）是一种转录因子，在细胞生长和癌症等许多细胞过程中发挥关键作用。然而，STAT3调节细胞过程的功能和机制尚未完全了解。

结果

在此我们描述了STAT3的一种新功能。我们证明STAT3在DNA复制中起重要作用。具体而言，敲低STAT3会减少DNA复制，而激活和异位表达STAT3则会促进DNA复制。我们进一步鉴定出WD重复和HMG盒DNA结合蛋白1（WDHD1），其在DNA复制起始中起重要作用，是介导STAT3的DNA复制功能的一个新的STAT3靶基因。我们表明STAT3结合WDHD1基因的启动子/上调区域。