Department of Emergency, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe Road, Zhengzhou City, Henan 450052, China.
Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe Road, Zhengzhou City, Henan 450052, China.
Oxid Med Cell Longev. 2020 Dec 13;2020:8189706. doi: 10.1155/2020/8189706. eCollection 2020.
Intervertebral disc degeneration (IDD) is a prevalent disease characterized by low back pain. Increasing extracellular matrix (ECM) synthesis and decreasing nucleus pulposus cell (NPC) apoptosis are promising strategies to recover degenerated NP. LIM mineralization protein- (LMP-) 1 has anti-inflammatory potential and is a promising gene target for the treatment of NP degeneration. In this study, we measured the expression of LMP-1 in the NP of patients. Then, we constructed LMP-1-overexpressing NPCs using lentiviral vectors and investigated the effects of LMP-1 on cell proliferation, apoptosis, and ECM synthesis in NPCs. The results showed that LMP-1 was highly expressed in the NP of patients. LMP-1 overexpression significantly increased proliferation and decreased apoptosis in NPCs. The expression of collagen II and sulfated glycosaminoglycan (sGAG) in NPCs was also upregulated after LMP-1 was overexpressed. Moreover, we demonstrated that LMP-1 decreased apoptosis of NPCs by inhibiting NF-B signaling activation. These findings suggest that LMP-1 plays an essential role in mediating apoptosis in NPCs by regulating NF-B signaling and can be used as a gene target for the treatment of IDD.
椎间盘退变（IDD）是一种常见疾病，以腰痛为特征。增加细胞外基质（ECM）的合成和减少髓核细胞（NPC）凋亡是恢复退变 NP 的有前途的策略。LIM 矿化蛋白-（LMP-）1 具有抗炎潜力，是治疗 NP 退变的有前途的基因靶点。在这项研究中，我们测量了 NP 中 LMP-1 在患者中的表达。然后，我们使用慢病毒载体构建了 LMP-1 过表达 NPC，并研究了 LMP-1 对 NPC 增殖、凋亡和 ECM 合成的影响。结果表明，LMP-1 在患者的 NP 中高度表达。LMP-1 过表达显着增加了 NPC 的增殖并减少了凋亡。LMP-1 过表达后，NPC 中胶原 II 和硫酸化糖胺聚糖（sGAG）的表达也上调。此外，我们证明 LMP-1 通过抑制 NF-B 信号激活来减少 NPC 的凋亡。这些发现表明，LMP-1 通过调节 NF-B 信号在 NPC 中介导凋亡中起重要作用，并可用作治疗 IDD 的基因靶点。
