Department of Obstetrics and Gynecology, Pelvic Floor Disease Diagnosis and Treatment Center, Shengjing Hospital of China Medical University, Shenyang City, Liaoning Province, China.
Arch Gynecol Obstet. 2021 May;303(5):1245-1253. doi: 10.1007/s00404-020-05913-8. Epub 2021 Jan 7.
To evaluate COX-2 and Nrf2/GPx3 expressions in the lamina propria of the anterior vaginal wall tissues of women with and without pelvic organ prolapse (POP).
Tissue samples of anterior vaginal wall were examined using HE staining, immuohistochemical staining and Western blot for the expressions of COX-2/PGE2, Nrf2/GPx3, MMP2, TIMP1, collagen I and collagen III (n = 35, per group).
Compared with control group, collagen fibers of the anterior vaginal wall were disorganized and discontinuous. Expressions of Nrf2, GPx3, TIMP1, collagen I and collagen III were found significantly lower in POP group (P < 0.05); while, expressions of COX-2, PGE2, and MMP2 were found significantly higher in POP group (P < 0.05). Statistically significant correlations of COX-2 and Nrf2/GPx3 were showed (P < 0.01).
We found that the interaction between inflammation and oxidative stress was closely related to the development of POP. This study demonstrates that COX-2 and Nrf2 pathways may be involved in pathogenesis of POP, as promising potential therapeutic targets and agents.
评估 COX-2 和 Nrf2/GPx3 在患有和不患有盆腔器官脱垂（POP）的女性前阴道壁组织固有层中的表达。
使用 HE 染色、免疫组织化学染色和 Western blot 检查前阴道壁组织样本，以检测 COX-2/PGE2、Nrf2/GPx3、MMP2、TIMP1、I 型和 III 型胶原蛋白的表达（每组 n=35）。
与对照组相比，POP 组前阴道壁的胶原纤维排列紊乱且不连续。POP 组 Nrf2、GPx3、TIMP1、I 型和 III 型胶原蛋白的表达明显降低（P<0.05）；而 COX-2、PGE2 和 MMP2 的表达明显升高（P<0.05）。COX-2 和 Nrf2/GPx3 之间存在显著的相关性（P<0.01）。
我们发现炎症和氧化应激之间的相互作用与 POP 的发展密切相关。本研究表明，COX-2 和 Nrf2 途径可能参与了 POP 的发病机制，为潜在的治疗靶点和药物提供了依据。
