Kim Y I, Salvini P, Auxilia F, Calne R Y
Department of Surgery, University of Cambridge, Addenbrookes Hospital, England.
Am J Surg. 1988 Feb;155(2):245-9. doi: 10.1016/s0002-9610(88)80705-0.
The effects of cyclosporin A on the regenerating liver were studied in rats after partial hepatectomy and were compared with those of conventional immunosuppressants such as methylprednisolone and azathioprine. In Group I, a standard hepatectomy removing two thirds of the organ was performed. In Group II, an oral dose of 10 mg/kg of cyclosporin A was given 1 day before and immediately, 24 hours, and 48 hours after the same hepatectomy. In Groups III and IV, azathioprine and methylprednisolone were administered in 8 mg/kg doses by way of the tail vein by the same protocol as in Group II. The mortality rate, liver weight restoration, and histologic and mitotic characteristics of the liver remnants, as well as serum levels of animotransferase, total bilirubin, and creatinine were examined from 24 hours to 10 days after the operation. Azathioprine and methylprednisolone substantially suppressed hepatocyte mitosis when compared with the control group; however, cyclosporin A treatment did not inhibit, but may, in fact, have stimulated liver cell proliferation. Changes in other parameters coincided with this phenomenon. The noninhibitory effect of cyclosporin A on liver regeneration has also been discussed.
在大鼠部分肝切除术后，研究了环孢素A对再生肝脏的影响，并与传统免疫抑制剂如甲泼尼龙和硫唑嘌呤的影响进行了比较。在第一组中，进行了切除三分之二肝脏的标准肝切除术。在第二组中，在相同肝切除术前1天以及术后立即、24小时和48小时口服10mg/kg的环孢素A。在第三组和第四组中，按照与第二组相同的方案通过尾静脉以8mg/kg的剂量给予硫唑嘌呤和甲泼尼龙。在术后24小时至10天期间，检查死亡率、肝脏重量恢复情况、肝残余组织的组织学和有丝分裂特征，以及血清转氨酶、总胆红素和肌酐水平。与对照组相比，硫唑嘌呤和甲泼尼龙显著抑制肝细胞有丝分裂；然而，环孢素A治疗并未抑制，实际上可能还刺激了肝细胞增殖。其他参数的变化与这一现象一致。还讨论了环孢素A对肝脏再生的非抑制作用。
