Department of Gastroenterology, Caoxian People's hospital, Heze, Shandong, China.
Department of Ultrasound, Yidu Central Hospital of Weifang, Weifang, Shandong, China.
Technol Cancer Res Treat. 2021 Jan-Dec;20:1533033820985858. doi: 10.1177/1533033820985858.
MicroRNA-1269 (miR-1269) has been identified upregulated in several cancers, as well as in esophageal cancer. In the present study, we investigated the clinical prognostic significance and potential functional role of miR-1269 in esophageal squamous cell carcinoma (ESCC).
A total of 107 ESCC patients who underwent surgical resection were enrolled in this study. miR-1269 expression was measured using quantitative real-time PCR (qRT-PCR). Kaplan-Meier method and multivariate Cox regression analysis were used to explore the prognostic significance of miR-1269. CCK-8 assays and Transwell assays were used to investigate the effects of miR-1269 on cell proliferation, migration, and invasion. The direct association between miR-1269 and SOX6 was evaluated using a dual-luciferase reporter assay.
The expression of miR-1269 was significantly upregulated in ESCC tissues and cell lines compared with adjacent normal tissues and esophageal epithelial cell line, respectively. What's more, the upregulation of miR-1269 was associated with positive lymph node metastasis and advanced TNM stage. ESCC patients with high miR-1269 expression had shorter overall survival than those with low miR-1269 expression levels. Compared with the control group, overexpression of miR-1269 promoted cell proliferation, migration, and invasion, while knockdown of miR-1269 inhibited cell proliferation, migration, and invasion. SOX6 was a direct target of miR-1269.
These results suggest that miR-1269 plays an important role in the progression of ESCC by targeting SOX6 and may be a potential prognostic biomarker and the miR-1269/SOX6 axis may be a therapeutic target for the patient with ESCC.
MicroRNA-1269（miR-1269）在多种癌症中上调，包括食管癌。在本研究中，我们研究了 miR-1269 在食管鳞状细胞癌（ESCC）中的临床预后意义和潜在功能作用。
本研究共纳入 107 例接受手术切除的 ESCC 患者。采用实时定量 PCR（qRT-PCR）测量 miR-1269 的表达。Kaplan-Meier 法和多因素 Cox 回归分析用于探讨 miR-1269 的预后意义。CCK-8 检测和 Transwell 检测用于研究 miR-1269 对细胞增殖、迁移和侵袭的影响。双荧光素酶报告基因检测用于评估 miR-1269 与 SOX6 之间的直接关联。
与相邻正常组织和食管上皮细胞系相比，miR-1269 在 ESCC 组织和细胞系中的表达显著上调。此外，miR-1269 的上调与阳性淋巴结转移和晚期 TNM 分期有关。miR-1269 高表达的 ESCC 患者总生存期短于 miR-1269 低表达水平的患者。与对照组相比，miR-1269 的过表达促进了细胞增殖、迁移和侵袭，而 miR-1269 的敲低则抑制了细胞增殖、迁移和侵袭。SOX6 是 miR-1269 的直接靶标。
这些结果表明，miR-1269 通过靶向 SOX6 在 ESCC 的进展中发挥重要作用，可能是潜在的预后生物标志物，miR-1269/SOX6 轴可能是 ESCC 患者的治疗靶点。
