Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of Ministry of Education, Shaanxi Key Laboratory of Land Consolidation, School of Water and Environment, Chang'an University, Xi'an 710054, PR China.
Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of Ministry of Education, Shaanxi Key Laboratory of Land Consolidation, School of Water and Environment, Chang'an University, Xi'an 710054, PR China.
Ecotoxicol Environ Saf. 2021 Mar 1;210:111878. doi: 10.1016/j.ecoenv.2020.111878. Epub 2021 Jan 5.
Flavonoids participate in several plant processes such as growth and physiological protection in adverse environments. In this study, we investigated the combined effects of eCO and cadmium (Cd)-contaminated soils on the total flavonoid and monomer contents in the leaves of Robinia pseudoacacia L. seedlings. Elevated CO, Cd, and eCO+ Cd increased the total flavonoids in the leaves relative to the control, and eCO mostly increased (p < 0.05) the total flavonoid content under Cd exposure. Elevated CO increased (p < 0.05) robinin, rutin, and acacetin contents in the leaves of 45-day seedlings and decreased (p < 0.05) the content of robinin and acacetin at 90 and 135 d under Cd exposure except for robinin at day 45 under Cd1 and acacetin on day 135 under Cd1. Quercetin content decreased (p < 0.05) under the combined conditions relative to Cd alone. Kaempferol in the leaves was only detected under eCO on day 135. The responses of total chlorophyll, total soluble sugars, starch, C, N, S, and the C/N ratio in the leaves to eCO significantly affected the synthesis of total flavonoids and monomers under Cd exposure. Overall, rutin was more sensitive to eCO+ Cd than the other flavonoids. Cadmium, CO and time had significant interactive effects on the synthesis of flavonoids in the leaves of R. pseudoacacia L. seedlings. Elevated CO may improve the protection and defense system of seedlings grown in Cd-contaminated soils by promoting the synthesis of total flavonoids, although robinin, rutin, quercetin, and acacetin yields may reduce with time. Additionally, increased Cd in the leaves suggested that eCO could improve the phytoremediation of Cd-contaminated soils.
类黄酮参与植物的多种过程，如生长和在不利环境中的生理保护。本研究调查了 eCO 和镉（Cd）污染土壤对刺槐幼苗叶片总黄酮和单体含量的综合影响。与对照相比，高 CO、Cd 和 eCO+Cd 增加了叶片中的总黄酮含量，而 eCO 在 Cd 暴露下大多增加（p<0.05）了总黄酮含量。高 CO 增加（p<0.05）了 45 天幼苗叶片中的罗伯宁、芦丁和芹菜素含量，降低（p<0.05）了 90 天和 135 天 Cd 暴露下罗伯宁和芹菜素的含量，但 Cd1 下 45 天的罗伯宁和 Cd1 下 135 天的芹菜素除外。在单独 Cd 存在下，槲皮素含量降低（p<0.05）。eCO 下仅在 135 天检测到叶片中的山柰酚。叶片中总叶绿素、总可溶性糖、淀粉、C、N、S 和 C/N 比的响应对 eCO 下 Cd 暴露时总黄酮和单体的合成有显著影响。总体而言，芦丁对 eCO+Cd 的敏感性比其他类黄酮更高。Cd、CO 和时间对刺槐幼苗叶片中黄酮类化合物的合成有显著的互作效应。高 CO 可能通过促进总黄酮的合成来改善幼苗在 Cd 污染土壤中的保护和防御系统，尽管随着时间的推移，罗伯宁、芦丁、槲皮素和芹菜素的产量可能会降低。此外，叶片中 Cd 的增加表明 eCO 可以提高 Cd 污染土壤的植物修复。
