Lv XiangGuo, Yang JingXuan, Feng Chao, Li Zhe, Chen ShiYan, Xie MinKai, Huang JianWen, Li HongBin, Wang HuaPing, Xu YueMin
Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai, China.
ACS Biomater Sci Eng. 2016 Jan 11;2(1):19-29. doi: 10.1021/acsbiomaterials.5b00259. Epub 2015 Dec 3.
In this study, we built a bilayer nanofibrous material by utilizing the gelatinization properties of potato starch (PS) to interrupt bacterial cellulose (BC) assembly during static culture to create more free spaces within the fibrous network. Then, muscle cells were cultured on the loose surface of the BC/PS scaffolds to build biomaterials for hollow organ reconstruction. Our results showed that the BC/PS scaffolds exhibited similar mechanical characters to those in the traditional BC scaffolds. And the pore sizes and porosities of BC/PS scaffolds could be controlled by adjusting the starch content. The average nanofiber diameters of unmodified BC and BC/PS composites is approximately to that of the urethral acellular matrix. Those scaffolds permit the muscle cells infiltration into the loose layer and the BC/PS membranes with muscle cells could enhance wound healing in vivo and vitro. Our study suggested that the use of bilayer BC/PS nanofibrous scaffolds may lead to improved vessel formation. BC/PS nanofibrous scaffolds with muscle cells enhanced the repair in dog urethral defect models, resulting in patent urethra. Improved organized muscle bundles and epithelial layer were observed in animals treated with BC/PS scaffold seeded by muscle cells compared with those treated with pure BC/PS scaffold. This study suggests that this biomaterial could be suitable for tissue engineered urinary tract reconstruction and this type of composite scaffold could be used for numerous other types of hollow organ tissue engineering grafts, including vascular, bladder, ureter, esophagus, and intestine.
在本研究中，我们利用马铃薯淀粉（PS）的糊化特性构建了一种双层纳米纤维材料，在静态培养过程中中断细菌纤维素（BC）的组装，以在纤维网络内创造更多自由空间。然后，将肌肉细胞培养在BC/PS支架的疏松表面上，构建用于中空器官重建的生物材料。我们的结果表明，BC/PS支架表现出与传统BC支架相似的力学特性。并且可以通过调节淀粉含量来控制BC/PS支架的孔径和孔隙率。未改性BC和BC/PS复合材料的平均纳米纤维直径与尿道脱细胞基质的直径相近。这些支架允许肌肉细胞浸润到疏松层中，带有肌肉细胞的BC/PS膜在体内和体外均可促进伤口愈合。我们的研究表明，使用双层BC/PS纳米纤维支架可能会改善血管形成。带有肌肉细胞的BC/PS纳米纤维支架增强了犬尿道缺损模型中的修复效果，形成了通畅的尿道。与用纯BC/PS支架治疗的动物相比，在用接种了肌肉细胞的BC/PS支架治疗的动物中观察到了更有序的肌肉束和上皮层。本研究表明，这种生物材料可能适用于组织工程化尿道重建，并且这种类型的复合支架可用于许多其他类型的中空器官组织工程移植物，包括血管、膀胱、输尿管、食管和肠道。
